Detailed information of TA system TA074793

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 93842..94081
• Replicon: plasmid unnamed1
• Accession: NZ_CP020496
• Organism: Escherichia coli strain 103

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: CPA47_RS26070
• Protein ID: WP_023144756.1
• Coordinates: 93842..93976 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	94021..94081 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPA47_RS26045	89080..89154	-	75	WP_031943482.1	RepA leader peptide Tap	-
CPA47_RS26890	89151..89285	-	135	Protein_96	protein CopA/IncA	-
CPA47_RS26050	89411..90624	+	1214	WP_162829202.1	IS3 family transposase	-
CPA47_RS26055	90829..92370	+	1542	WP_002431311.1	IS21-like element ISEc12 family transposase	-
CPA47_RS26060	92385..93131	+	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
CPA47_RS26065	93291..93545	-	255	WP_000083850.1	replication regulatory protein RepA	-
CPA47_RS26070	93842..93976	-	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	94021..94081	+	61	NuclAT_2	-	Antitoxin
-	94021..94081	+	61	NuclAT_2	-	Antitoxin
-	94021..94081	+	61	NuclAT_2	-	Antitoxin
-	94021..94081	+	61	NuclAT_2	-	Antitoxin
CPA47_RS26805	94048..94334	-	287	Protein_102	DUF2726 domain-containing protein	-
CPA47_RS26085	94847..95059	-	213	WP_013023861.1	hypothetical protein	-
CPA47_RS26090	95190..95750	-	561	WP_000139341.1	fertility inhibition protein FinO	-
CPA47_RS26095	95805..96551	-	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-
CPA47_RS26100	96571..99027	-	2457	Protein_106	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / dfrA17 / aadA5 / qacE / sul1 / blaTEM-1B / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / aac(3)-IId	-	1..120668	120668

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T74793 WP_023144756.1 NZ_CP020496:c93976-93842 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T74793 NZ_CP020496:c93976-93842 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT74793 NZ_CP020496:94021-94081 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin