Detailed information of TA system TA074785

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4956678..4957280
• Replicon: chromosome
• Accession: NZ_CP020495
• Organism: Escherichia coli strain 103

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: CPA47_RS24490
• Protein ID: WP_000897305.1
• Coordinates: 4956969..4957280 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CPA47_RS24485
• Protein ID: WP_000356395.1
• Coordinates: 4956678..4956968 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPA47_RS24445	4952301..4953203	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
CPA47_RS24450	4953200..4953835	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CPA47_RS24455	4953832..4954761	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
CPA47_RS24460	4954943..4955185	-	243	WP_021523315.1	hypothetical protein	-
CPA47_RS24465	4955404..4955622	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
CPA47_RS24475	4956041..4956319	-	279	WP_001306650.1	hypothetical protein	-
CPA47_RS24485	4956678..4956968	-	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
CPA47_RS24490	4956969..4957280	-	312	WP_000897305.1	hypothetical protein	Toxin
CPA47_RS24495	4957509..4958417	+	909	WP_001306651.1	alpha/beta hydrolase	-
CPA47_RS24500	4958586..4960400	-	1815	WP_020233236.1	hypothetical protein	-
CPA47_RS24505	4960816..4961757	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CPA47_RS24510	4961802..4962239	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T74785 WP_000897305.1 NZ_CP020495:c4957280-4956969 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T74785 NZ_CP020495:c4957280-4956969 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT74785 WP_000356395.1 NZ_CP020495:c4956968-4956678 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT74785 NZ_CP020495:c4956968-4956678 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7