Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3993304..3993998 Replicon chromosome
Accession NZ_CP020495
Organism Escherichia coli strain 103

Toxin (Protein)


Gene name yafO Uniprot ID A0A0D8WHS4
Locus tag CPA47_RS19785 Protein ID WP_001521903.1
Coordinates 3993304..3993702 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag CPA47_RS19790 Protein ID WP_000554758.1
Coordinates 3993705..3993998 (-) Length 98 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPA47_RS19755 3988474..3989718 - 1245 WP_000189541.1 esterase FrsA -
- 3989134..3989214 - 81 NuclAT_11 - -
- 3989134..3989214 - 81 NuclAT_11 - -
- 3989134..3989214 - 81 NuclAT_11 - -
- 3989134..3989214 - 81 NuclAT_11 - -
CPA47_RS19760 3989810..3990268 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
CPA47_RS19765 3990529..3991986 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
CPA47_RS26855 3992043..3992395 - 353 Protein_3794 peptide chain release factor H -
CPA47_RS19775 3992391..3992597 - 207 Protein_3795 RtcB family protein -
CPA47_RS19780 3992842..3993294 - 453 WP_023144376.1 GNAT family N-acetyltransferase -
CPA47_RS19785 3993304..3993702 - 399 WP_001521903.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
CPA47_RS19790 3993705..3993998 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
CPA47_RS19795 3994050..3995105 - 1056 WP_001226177.1 DNA polymerase IV -
CPA47_RS19800 3995176..3995961 - 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
CPA47_RS19805 3995933..3997645 + 1713 Protein_3801 flagellar biosynthesis protein FlhA -
CPA47_RS19810 3997724..3998077 + 354 WP_024166466.1 type II toxin-antitoxin system HicB family antitoxin -
CPA47_RS19815 3998134..3998883 - 750 WP_016233951.1 C40 family peptidase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3983241..3993998 10757


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-130)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 133 a.a.        Molecular weight: 15471.88 Da        Isoelectric Point: 8.0949

>T74778 WP_001521903.1 NZ_CP020495:c3993702-3993304 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T74778 NZ_CP020495:c3993702-3993304 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT74778 WP_000554758.1 NZ_CP020495:c3993998-3993705 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT74778 NZ_CP020495:c3993998-3993705 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0D8WHS4


Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References