Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3169278..3169780 Replicon chromosome
Accession NZ_CP020495
Organism Escherichia coli strain 103

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CPA47_RS15905 Protein ID WP_000767829.1
Coordinates 3169278..3169532 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CPA47_RS15910 Protein ID WP_001259255.1
Coordinates 3169529..3169780 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPA47_RS15875 3164293..3164520 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
CPA47_RS15880 3164534..3165592 - 1059 WP_000492324.1 transport protein YeeE -
CPA47_RS15885 3165771..3167129 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CPA47_RS15890 3167119..3167181 - 63 WP_010723108.1 membrane protein YoeI -
CPA47_RS15895 3167396..3168325 - 930 WP_023154044.1 LysR family transcriptional regulator -
CPA47_RS15900 3168371..3169195 - 825 WP_000754758.1 SDR family oxidoreductase -
CPA47_RS15905 3169278..3169532 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CPA47_RS15910 3169529..3169780 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CPA47_RS15915 3170063..3170113 + 51 WP_001364200.1 his operon leader peptide -
CPA47_RS15920 3170259..3171158 + 900 WP_021523143.1 ATP phosphoribosyltransferase -
CPA47_RS15925 3171164..3172468 + 1305 WP_021523144.1 histidinol dehydrogenase -
CPA47_RS15930 3172465..3173535 + 1071 WP_000108937.1 histidinol-phosphate transaminase -
CPA47_RS15935 3173535..3174602 + 1068 WP_000080136.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T74774 WP_000767829.1 NZ_CP020495:c3169532-3169278 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T74774 NZ_CP020495:c3169532-3169278 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT74774 WP_001259255.1 NZ_CP020495:c3169780-3169529 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT74774 NZ_CP020495:c3169780-3169529 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References