Detailed information of TA system TA074759

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2392073..2392444
• Replicon: chromosome
• Accession: NZ_CP020495
• Organism: Escherichia coli strain 103

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: CPA47_RS11865
• Protein ID: WP_001443846.1
• Coordinates: 2392295..2392444 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2392073..2392251 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPA47_RS11840	2388028..2389401	+	1374	WP_000123748.1	ATP-dependent RNA helicase DbpA	-
CPA47_RS11845	2389530..2390465	-	936	WP_001523172.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
CPA47_RS11850	2390517..2391752	-	1236	WP_000040839.1	site-specific integrase	-
CPA47_RS11855	2391754..2391969	-	216	WP_000079604.1	excisionase XisR	-
CPA47_RS11860	2392069..2392257	-	189	WP_001302840.1	DUF1187 family protein	-
-	2392073..2392251	+	179	NuclAT_0	-	Antitoxin
-	2392073..2392251	+	179	NuclAT_0	-	Antitoxin
-	2392073..2392251	+	179	NuclAT_0	-	Antitoxin
-	2392073..2392251	+	179	NuclAT_0	-	Antitoxin
CPA47_RS11865	2392295..2392444	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
CPA47_RS11870	2392500..2393309	-	810	WP_000166313.1	recombination protein RecT	-
CPA47_RS11875	2393302..2395902	-	2601	WP_000105140.1	exodeoxyribonuclease VIII	-
CPA47_RS11880	2396004..2396279	-	276	WP_001344816.1	hypothetical protein	-
CPA47_RS11885	2396354..2396524	-	171	WP_001352098.1	conserved protein, Rac prophage	-
CPA47_RS11890	2396524..2396745	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2385704..2440945	55241

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T74759 WP_001443846.1 NZ_CP020495:c2392444-2392295 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T74759 NZ_CP020495:c2392444-2392295 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT74759 NZ_CP020495:2392073-2392251 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin