Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 80815..81350 Replicon plasmid unnamed1
Accession NZ_CP020489
Organism Enterococcus faecium strain CFSAN059071

Toxin (Protein)


Gene name yoeB Uniprot ID R2PQC0
Locus tag B6S06_RS15295 Protein ID WP_002314366.1
Coordinates 80815..81087 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q3Y220
Locus tag B6S06_RS15300 Protein ID WP_002297422.1
Coordinates 81087..81350 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B6S06_RS15250 75921..76370 - 450 WP_010729386.1 TlpA family protein disulfide reductase -
B6S06_RS16550 76526..77068 - 543 WP_002298088.1 hypothetical protein -
B6S06_RS15265 77343..77986 + 644 Protein_78 transposase -
B6S06_RS15270 78062..78742 + 681 WP_002331383.1 IS6-like element IS1216 family transposase -
B6S06_RS15275 78801..78989 - 189 Protein_80 hypothetical protein -
B6S06_RS15280 78990..79847 - 858 WP_002292680.1 AAA family ATPase -
B6S06_RS15285 80133..80419 - 287 Protein_82 type II toxin-antitoxin system RelE/ParE family toxin -
B6S06_RS15290 80409..80738 - 330 WP_002292678.1 AbrB family transcriptional regulator -
B6S06_RS15295 80815..81087 - 273 WP_002314366.1 Txe/YoeB family addiction module toxin Toxin
B6S06_RS15300 81087..81350 - 264 WP_002297422.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
B6S06_RS15305 81584..82492 + 909 Protein_86 IS982 family transposase -
B6S06_RS15310 82495..83670 - 1176 WP_002336205.1 MFS transporter -
B6S06_RS15315 83673..85922 - 2250 WP_002314387.1 alpha-galactosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..217014 217014
- inside IScluster/Tn - - 69252..93842 24590


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10780.24 Da        Isoelectric Point: 9.9724

>T74745 WP_002314366.1 NZ_CP020489:c81087-80815 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T74745 NZ_CP020489:c81087-80815 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9959.22 Da        Isoelectric Point: 4.5370

>AT74745 WP_002297422.1 NZ_CP020489:c81350-81087 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT74745 NZ_CP020489:c81350-81087 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F704

Antitoxin

Source ID Structure
AlphaFold DB A0A829F006

References