Detailed information of TA system TA074652

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 761419..762058
• Replicon: chromosome
• Accession: NZ_CP020411
• Organism: Haemophilus influenzae strain FDAARGOS_199

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8B2WM63
• Locus tag: A6J38_RS03770
• Protein ID: WP_005694311.1
• Coordinates: 761753..762058 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A6J38_RS03765
• Protein ID: WP_005650217.1
• Coordinates: 761419..761742 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6J38_RS09145	757038..757211	-	174	WP_005652999.1	DUF5363 domain-containing protein	-
A6J38_RS03750	757208..759178	-	1971	WP_005694313.1	tRNA(Met) cytidine acetyltransferase TmcA	-
A6J38_RS03755	759183..759524	-	342	WP_005666735.1	SirB2 family protein	-
A6J38_RS03760	759586..761256	-	1671	WP_005650218.1	energy-dependent translational throttle protein EttA	-
A6J38_RS03765	761419..761742	-	324	WP_005650217.1	HigA family addiction module antidote protein	Antitoxin
A6J38_RS03770	761753..762058	-	306	WP_005694311.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A6J38_RS03775	762383..763003	+	621	WP_005694310.1	zinc transporter binding subunit ZevA	-
A6J38_RS03780	763006..763974	+	969	WP_005694309.1	zinc transporter permease subunit ZevB	-
A6J38_RS03790	764588..766627	+	2040	WP_005694308.1	excinuclease ABC subunit B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12248.89 Da
• Isoelectric Point: 8.0244

>T74652 WP_005694311.1 NZ_CP020411:c762058-761753 [Haemophilus influenzae]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYCL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T74652 NZ_CP020411:c762058-761753 [Haemophilus influenzae]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCCATTCGAGTGAATAAACAATATTGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT74652 WP_005650217.1 NZ_CP020411:c761742-761419 [Haemophilus influenzae]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT74652 NZ_CP020411:c761742-761419 [Haemophilus influenzae]
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACGCTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAGCCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A8B2WM63