Detailed information of TA system TA074606

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1339767..1340478
• Replicon: chromosome
• Accession: NZ_CP020403
• Organism: Pasteurella multocida strain FDAARGOS_216

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2J9QLH7
• Locus tag: A6J55_RS06365
• Protein ID: WP_005725755.1
• Coordinates: 1340152..1340478 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A6J55_RS06360
• Protein ID: WP_005757699.1
• Coordinates: 1339767..1340093 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6J55_RS06340	1335178..1336236	+	1059	WP_005755108.1	hypothetical protein	-
A6J55_RS06345	1336474..1337481	+	1008	WP_005755103.1	hypothetical protein	-
A6J55_RS06350	1337532..1338134	+	603	WP_005755098.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
A6J55_RS06355	1338193..1339713	+	1521	WP_005755096.1	DUF560 domain-containing protein	-
A6J55_RS06360	1339767..1340093	-	327	WP_005757699.1	HigA family addiction module antidote protein	Antitoxin
A6J55_RS06365	1340152..1340478	-	327	WP_005725755.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A6J55_RS06370	1340636..1341112	+	477	WP_005755093.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
A6J55_RS06375	1341187..1342056	-	870	WP_005755091.1	VirK/YbjX family protein	-
A6J55_RS06380	1342167..1342784	+	618	WP_005755089.1	ribosome biogenesis GTP-binding protein YihA/YsxC	-
A6J55_RS06385	1342892..1344421	+	1530	WP_005755087.1	YifB family Mg chelatase-like AAA ATPase	-
A6J55_RS06390	1344475..1345161	-	687	WP_005755085.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (12-104)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-102)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12892.73 Da
• Isoelectric Point: 9.6705

>T74606 WP_005725755.1 NZ_CP020403:c1340478-1340152 [Pasteurella multocida]
MDRVRFNLTEDSFQDKYLYEYFLYGSKHKNIPSDIENTLAHKLDMLDSANTLSDLRSPPGNKLEKLEPKTSGLYSIRVNV
KYRLIFKYKESNFPKITELRLDKHQYRL

Nucleotide

• Length: 327 bp

>T74606 NZ_CP020403:c1340478-1340152 [Pasteurella multocida]
ATGGACAGAGTCCGATTTAATCTTACGGAAGATAGCTTCCAAGACAAGTATCTGTATGAGTATTTTTTATATGGAAGTAA
GCATAAAAATATTCCAAGTGATATAGAAAATACGCTAGCGCATAAATTAGATATGTTAGATAGCGCAAATACTTTGAGTG
ATTTACGAAGTCCACCTGGTAATAAATTGGAAAAATTGGAGCCTAAAACGAGTGGGCTATATTCTATCAGAGTGAATGTG
AAGTATCGTTTAATATTTAAATATAAAGAAAGTAATTTTCCAAAAATAACAGAGTTGAGATTAGATAAACATCAATATAG
ATTATAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12377.41 Da
• Isoelectric Point: 7.2034

>AT74606 WP_005757699.1 NZ_CP020403:c1340093-1339767 [Pasteurella multocida]
MSRVQRKPSTVGDILLDEYLVPLNLKIADLATMLDVHRNTASALVNNNTKLSLEMALKLAKLFNTSPEFWLNLQMKLDLW
EIENNARFQESLKKISSIDQHNLLEKIA

Nucleotide

• Length: 327 bp

>AT74606 NZ_CP020403:c1340093-1339767 [Pasteurella multocida]
ATGAGTAGAGTACAAAGAAAGCCATCAACAGTGGGTGATATTTTATTAGATGAATATTTAGTTCCACTAAATTTGAAAAT
TGCAGATTTAGCTACAATGTTAGATGTTCATCGTAATACAGCAAGTGCACTCGTTAATAATAATACTAAATTGTCTTTAG
AAATGGCACTGAAACTAGCAAAACTATTTAATACAAGTCCTGAGTTTTGGTTAAATTTACAAATGAAATTAGACTTATGG
GAAATTGAAAACAATGCTAGGTTTCAAGAAAGTTTAAAGAAAATTTCGTCTATTGATCAGCATAATCTGTTAGAAAAAAT
CGCTTGA

Structures

Toxin

• AlphaFold DB: A0A2J9QLH7