Detailed information of TA system TA074563

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 1328996..1329645
• Replicon: chromosome
• Accession: NZ_CP020388
• Organism: Pluralibacter gergoviae strain FDAARGOS_186

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1S2BAV2
• Locus tag: A8H26_RS06435
• Protein ID: WP_048254040.1
• Coordinates: 1328996..1329337 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A8H26_RS06440
• Protein ID: WP_048253972.1
• Coordinates: 1329334..1329645 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8H26_RS06410	1324362..1325804	-	1443	WP_048253975.1	PTS N-acetylmuramic acid transporter subunit IIBC	-
A8H26_RS06415	1325807..1326718	-	912	WP_048253974.1	N-acetylmuramic acid 6-phosphate etherase	-
A8H26_RS06420	1326835..1327722	-	888	WP_048254041.1	LysR family transcriptional regulator	-
A8H26_RS06425	1327825..1328235	+	411	WP_048253973.1	CidA/LrgA family protein	-
A8H26_RS06430	1328228..1328917	+	690	WP_043081728.1	LrgB family protein	-
A8H26_RS06435	1328996..1329337	+	342	WP_048254040.1	hypothetical protein	Toxin
A8H26_RS06440	1329334..1329645	+	312	WP_048253972.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
A8H26_RS06445	1329685..1331334	-	1650	WP_106912782.1	cation/acetate symporter ActP	-
A8H26_RS06450	1331334..1331645	-	312	WP_043081730.1	DUF485 domain-containing protein	-
A8H26_RS06455	1331868..1333826	-	1959	WP_048253970.1	acetate--CoA ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Antitoxin MqsA/HigA-2 (30-99)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13422.37 Da
• Isoelectric Point: 9.7299

>T74563 WP_048254040.1 NZ_CP020388:1328996-1329337 [Pluralibacter gergoviae]
MDALFIELPPFERHRAEYFSDDDFKAFQQMLLHNPCAGDVIRGAGGLRKIRFADPRRNKGKRGGIRVIYYWYAEKSHFLL
FTLYDKEQQDDLTAHQRNILSHLLEQAKQRIIR

Nucleotide

• Length: 342 bp

>T74563 NZ_CP020388:1328996-1329337 [Pluralibacter gergoviae]
ATGGATGCGCTGTTTATTGAACTACCGCCCTTTGAACGGCATCGTGCTGAATACTTCAGCGATGATGATTTCAAGGCTTT
CCAGCAAATGTTGTTGCATAATCCTTGCGCCGGGGATGTCATTCGAGGCGCCGGGGGACTTCGTAAAATTCGATTTGCTG
ACCCTCGCAGGAATAAGGGGAAGCGGGGAGGTATTCGGGTTATCTATTACTGGTATGCCGAGAAGTCCCATTTTCTGCTT
TTTACGCTATACGATAAAGAGCAGCAGGATGATTTGACGGCTCACCAACGTAACATCCTGTCCCATCTTTTAGAGCAGGC
AAAACAGAGGATCATCAGGTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11596.36 Da
• Isoelectric Point: 10.0436

>AT74563 WP_048253972.1 NZ_CP020388:1329334-1329645 [Pluralibacter gergoviae]
VTKRDIFSELQEGIEAWGELNAGKKTLRTHRVNTRDLAIAPEDLVKVREQLNLSQAVFARYLHAGLKTYQNWEQGLASPN
KQAVLLIRMIEKSPSVLSQLAAI

Nucleotide

• Length: 312 bp

>AT74563 NZ_CP020388:1329334-1329645 [Pluralibacter gergoviae]
GTGACGAAGCGAGATATTTTCAGCGAATTGCAGGAAGGTATTGAAGCGTGGGGCGAGTTAAATGCCGGAAAAAAAACACT
GCGCACTCACCGCGTTAATACCCGCGATCTGGCGATTGCGCCGGAAGATTTGGTCAAGGTTCGTGAACAGCTCAATCTTT
CGCAGGCCGTTTTCGCGCGCTATCTTCATGCGGGCCTGAAGACTTACCAGAATTGGGAGCAGGGCCTTGCCAGTCCAAAC
AAACAGGCGGTGCTATTGATCCGCATGATTGAGAAAAGTCCCTCTGTATTGTCTCAGCTTGCCGCTATCTAA

Structures

Toxin

• AlphaFold DB: A0A1S2BAV2