Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3565471..3566141 Replicon chromosome
Accession NZ_CP020381
Organism Mycobacterium tuberculosis strain MTB1

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag B5817_RS17480 Protein ID WP_003899954.1
Coordinates 3565471..3565815 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag B5817_RS17485 Protein ID WP_003899955.1
Coordinates 3565812..3566141 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B5817_RS17445 3560544..3561404 + 861 WP_003416628.1 alpha/beta hydrolase -
B5817_RS17450 3561379..3561894 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
B5817_RS17460 3562134..3562427 + 294 WP_003416635.1 hypothetical protein -
B5817_RS17465 3562715..3564004 + 1290 WP_031664058.1 ATP-binding protein -
B5817_RS17470 3564351..3564785 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
B5817_RS17475 3564788..3565240 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
B5817_RS17480 3565471..3565815 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
B5817_RS17485 3565812..3566141 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
B5817_RS17490 3566679..3567026 + 348 WP_003899956.1 DUF2384 domain-containing protein -
B5817_RS17495 3567023..3567643 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
B5817_RS17500 3567803..3569068 - 1266 Protein_3310 hypothetical protein -
B5817_RS17505 3569236..3569445 + 210 WP_003416778.1 hypothetical protein -
B5817_RS17515 3569692..3570726 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T74547 WP_003899954.1 NZ_CP020381:3565471-3565815 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T74547 NZ_CP020381:3565471-3565815 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT74547 WP_003899955.1 NZ_CP020381:3565812-3566141 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT74547 NZ_CP020381:3565812-3566141 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References