Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) unclassified/-
Location 2976132..2976780 Replicon chromosome
Accession NZ_CP020381
Organism Mycobacterium tuberculosis strain MTB1

Toxin (Protein)


Gene name novel[antitoxin] Uniprot ID P9WJ12
Locus tag B5817_RS14530 Protein ID WP_003899414.1
Coordinates 2976132..2976455 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name novel[toxin] Uniprot ID P9WJ10
Locus tag B5817_RS14535 Protein ID WP_003899415.1
Coordinates 2976535..2976780 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B5817_RS14500 2971455..2972453 + 999 WP_003900538.1 tyrosine-type recombinase/integrase -
B5817_RS14505 2972467..2972931 + 465 WP_003900539.1 ParB N-terminal domain-containing protein -
B5817_RS14510 2972919..2973170 + 252 WP_003908028.1 hypothetical protein -
B5817_RS14515 2973341..2974780 - 1440 WP_003899411.1 phage major capsid protein -
B5817_RS14520 2974788..2975321 - 534 WP_003899412.1 HK97 family phage prohead protease -
B5817_RS14525 2975474..2975965 - 492 WP_003900541.1 phage terminase small subunit P27 family -
B5817_RS14530 2976132..2976455 - 324 WP_003899414.1 type II toxin-antitoxin system toxin Toxin
B5817_RS14535 2976535..2976780 - 246 WP_003899415.1 type II toxin-antitoxin system antitoxin Antitoxin
B5817_RS14540 2976777..2978204 - 1428 WP_031664026.1 DUF3631 domain-containing protein -
B5817_RS14545 2978206..2978598 - 393 WP_003899417.1 DUF2742 domain-containing protein -
B5817_RS14550 2978595..2978855 - 261 WP_003899418.1 helix-turn-helix domain-containing protein -
B5817_RS14555 2978872..2979234 - 363 WP_003900543.1 hypothetical protein -
B5817_RS14560 2979237..2980364 - 1128 WP_003899420.1 site-specific integrase -
B5817_RS14565 2980509..2980736 - 228 WP_003899421.1 hypothetical protein -
B5817_RS14570 2980733..2981122 - 390 WP_003899422.1 hypothetical protein -
B5817_RS14575 2981028..2981300 + 273 WP_003900544.1 hypothetical protein -
B5817_RS14580 2981399..2981632 + 234 WP_003413717.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2971455..2980364 8909


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-126)

Antitoxin

SpoVT-AbrB domain

(6-43)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12359.82 Da        Isoelectric Point: 8.6717

>T74539 WP_003899414.1 NZ_CP020381:c2976455-2976132 [Mycobacterium tuberculosis]
MTHKRTKRQPAIAAGLNAPRRNRVGRQHGWPADVPSAEQRRAQRQRDLEAIRRAYAEMVATSHEIDDDTAELALLSMHLD
DEQRRLEAGMKLGWHPYHFPDEPDSKQ

Download         Length: 324 bp

>T74539 NZ_CP020381:c2976455-2976132 [Mycobacterium tuberculosis]
TTGACCCACAAGCGCACTAAACGCCAGCCAGCCATCGCCGCAGGGCTCAACGCCCCGCGTCGGAATCGCGTTGGGCGGCA
ACATGGTTGGCCGGCCGACGTTCCGTCCGCCGAGCAGCGCCGCGCCCAACGGCAGCGCGACCTCGAGGCTATCCGCCGAG
CGTACGCCGAGATGGTGGCGACATCACACGAAATCGACGACGACACAGCCGAACTGGCGCTGTTGTCGATGCATCTCGAC
GATGAGCAGCGCCGGCTTGAGGCGGGGATGAAGCTCGGCTGGCATCCGTATCACTTCCCCGACGAACCCGACAGCAAACA
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 7697.71 Da        Isoelectric Point: 4.8565

>AT74539 WP_003899415.1 NZ_CP020381:c2976780-2976535 [Mycobacterium tuberculosis]
MSGHALAARTLLAAADELVGGPPVEASAAALAGDAAGAWRTAAVELARALVRAVAESHGVAAVLFAATAAAAAAVDRGDP
P

Download         Length: 246 bp

>AT74539 NZ_CP020381:c2976780-2976535 [Mycobacterium tuberculosis]
ATGAGCGGCCACGCGTTGGCTGCTCGGACGTTGCTGGCCGCCGCGGACGAGCTTGTCGGCGGCCCGCCAGTCGAGGCTTC
GGCCGCCGCGCTGGCCGGCGACGCCGCGGGCGCATGGCGGACCGCGGCCGTCGAGCTTGCGCGAGCGTTGGTCCGCGCTG
TGGCGGAGTCGCACGGCGTCGCGGCCGTTTTGTTCGCCGCGACGGCCGCCGCGGCGGCGGCCGTCGACCGGGGTGATCCG
CCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045IHC4

Antitoxin

Source ID Structure
AlphaFold DB A0A806JR81

References