Detailed information of TA system TA074333

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 29363..30035
• Replicon: chromosome
• Accession: NZ_CP020350
• Organism: Pectobacterium brasiliense strain SX309

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: B5S52_RS00140
• Protein ID: WP_080162852.1
• Coordinates: 29649..30035 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A433N287
• Locus tag: B5S52_RS00135
• Protein ID: WP_010281167.1
• Coordinates: 29363..29662 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B5S52_RS00115	24628..25605	+	978	WP_039511567.1	nucleoside hydrolase	-
B5S52_RS00120	25605..26582	+	978	WP_010296900.1	ABC transporter permease	-
B5S52_RS00125	26579..27487	+	909	WP_040035635.1	ABC transporter permease	-
B5S52_RS00130	27527..29326	+	1800	WP_080162850.1	ABC transporter ATP-binding protein	-
B5S52_RS00135	29363..29662	-	300	WP_010281167.1	XRE family transcriptional regulator	Antitoxin
B5S52_RS00140	29649..30035	-	387	WP_080162852.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B5S52_RS00145	30357..31055	+	699	WP_005976717.1	D-galactonate utilization transcriptional regulator DgoR	-
B5S52_RS00150	31052..31948	+	897	WP_039521329.1	2-dehydro-3-deoxygalactonokinase	-
B5S52_RS00155	31932..32549	+	618	WP_010281152.1	2-dehydro-3-deoxy-6-phosphogalactonate aldolase	-
B5S52_RS00160	32546..33694	+	1149	WP_010281150.1	galactonate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-113)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14837.80 Da
• Isoelectric Point: 4.6497

>T74333 WP_080162852.1 NZ_CP020350:c30035-29649 [Pectobacterium brasiliense]
MEVYRLAWEIVTRALFDTWFDEQTETVQEEILAHLAILEEDDPQLGRPQVDQIKGSKYKNMKELRVQVGGHPFRTFFAFD
RTRKAIVLCAGDKKGEDEKLFYERMIKIADAEFASHLTSPEVKEDGDA

Nucleotide

• Length: 387 bp

>T74333 NZ_CP020350:c30035-29649 [Pectobacterium brasiliense]
ATGGAGGTTTACAGATTGGCATGGGAAATAGTCACACGGGCACTATTCGATACGTGGTTTGATGAGCAAACTGAAACGGT
ACAGGAAGAGATTTTGGCACACCTCGCTATACTCGAAGAGGATGACCCACAGCTAGGGCGACCACAGGTTGATCAGATTA
AAGGCTCAAAATACAAAAATATGAAGGAGCTTCGAGTTCAGGTTGGCGGCCACCCATTCCGAACATTCTTCGCGTTTGAC
CGGACACGTAAAGCGATTGTTTTATGTGCAGGAGATAAGAAAGGCGAAGATGAAAAGTTGTTCTACGAACGTATGATTAA
GATCGCAGACGCTGAGTTTGCATCGCATCTGACATCACCGGAGGTAAAAGAAGATGGCGACGCTTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11048.08 Da
• Isoelectric Point: 10.6442

>AT74333 WP_010281167.1 NZ_CP020350:c29662-29363 [Pectobacterium brasiliense]
MATLKEMMAKRSPESRARIEARTAEIRQEITLAKLREELNMSQTQLALALGVKQPSIVKMEKVENDPKLSTLKRYVTALG
GKLTLDVTLPDGKRIALTL

Nucleotide

• Length: 300 bp

>AT74333 NZ_CP020350:c29662-29363 [Pectobacterium brasiliense]
ATGGCGACGCTTAAAGAAATGATGGCTAAACGTTCACCGGAGAGCCGCGCTCGCATTGAAGCGCGCACGGCTGAGATCCG
TCAGGAAATCACTTTAGCGAAATTACGCGAAGAGCTGAACATGTCGCAGACTCAGCTTGCGTTAGCATTGGGCGTCAAGC
AGCCATCTATTGTTAAGATGGAGAAGGTTGAAAATGACCCAAAACTGTCAACGCTGAAACGCTATGTCACTGCCCTAGGC
GGCAAGCTTACGCTAGATGTGACCTTGCCTGATGGTAAGCGTATCGCGCTTACTTTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A433N287