Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 4615512..4616356 Replicon chromosome
Accession NZ_CP020342
Organism Shigella flexneri 1a strain 0439

Toxin (Protein)


Gene name itaT Uniprot ID -
Locus tag BS647_RS25040 Protein ID WP_014532172.1
Coordinates 4615895..4616356 (+) Length 154 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID A5H0K0
Locus tag BS647_RS25030 Protein ID WP_005053053.1
Coordinates 4615512..4615823 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BS647_RS25005 (4610983) 4610983..4611480 + 498 Protein_4532 COX aromatic rich motif-containing protein -
BS647_RS25010 (4611502) 4611502..4613493 + 1992 Protein_4533 cytochrome o ubiquinol oxidase subunit I -
BS647_RS25015 (4613483) 4613483..4614097 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
BS647_RS25020 (4614097) 4614097..4614426 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
BS647_RS25025 (4614438) 4614438..4615328 + 891 WP_000971346.1 heme o synthase -
BS647_RS25030 (4615512) 4615512..4615823 + 312 WP_005053053.1 DUF1778 domain-containing protein Antitoxin
BS647_RS25040 (4615895) 4615895..4616356 + 462 WP_014532172.1 GNAT family N-acetyltransferase Toxin
BS647_RS25050 (4617252) 4617252..4619405 + 2154 WP_005060811.1 SEL1-like repeat protein -
BS647_RS25055 (4619458) 4619458..4621188 + 1731 WP_000645013.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 4616404..4616907 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-133)

Antitoxin

Vibrio phage ICP1, Orf50

(15-97)


Sequences

Toxin        


Download         Length: 154 a.a.        Molecular weight: 16623.52 Da        Isoelectric Point: 9.9538

>T74328 WP_014532172.1 NZ_CP020342:4615895-4616356 [Shigella flexneri 1a]
MIAVTLLSINLYALRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVMLGGAR
KYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFYARLGFVQLSATPNAFGAVPMFLAIQHILAA

Download         Length: 462 bp

>T74328 NZ_CP020342:4615895-4616356 [Shigella flexneri 1a]
TTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACTG
TGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAGAC
AACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGGAGCGCGG
AAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAAAAATTCACCCGGCATTACC
AATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCGA
CACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11394.07 Da        Isoelectric Point: 10.3388

>AT74328 WP_005053053.1 NZ_CP020342:4615512-4615823 [Shigella flexneri 1a]
MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT74328 NZ_CP020342:4615512-4615823 [Shigella flexneri 1a]
ATGCCTGGAAAAACAGCCGCTCTATACGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A5H0K0

References