Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4578556..4579174 Replicon chromosome
Accession NZ_CP020342
Organism Shigella flexneri 1a strain 0439

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag BS647_RS24835 Protein ID WP_001291435.1
Coordinates 4578956..4579174 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q0T7C3
Locus tag BS647_RS24830 Protein ID WP_000344797.1
Coordinates 4578556..4578930 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BS647_RS24805 (4574078) 4574078..4575271 + 1194 WP_011069267.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
BS647_RS24810 (4575294) 4575294..4575512 + 219 Protein_4495 efflux RND transporter permease subunit -
BS647_RS24815 (4575546) 4575546..4576937 - 1392 WP_001493761.1 ISKra4-like element ISKpn19 family transposase -
BS647_RS24820 (4576974) 4576974..4577546 - 573 WP_001493762.1 recombinase family protein -
BS647_RS24825 (4577683) 4577683..4578273 - 591 WP_012477564.1 plasmid pRiA4b ORF-3 family protein -
BS647_RS24830 (4578556) 4578556..4578930 + 375 WP_000344797.1 Hha toxicity modulator TomB Antitoxin
BS647_RS24835 (4578956) 4578956..4579174 + 219 WP_001291435.1 HHA domain-containing protein Toxin
BS647_RS24840 (4579346) 4579346..4579897 + 552 Protein_4501 maltose O-acetyltransferase -
BS647_RS24845 (4580013) 4580013..4580483 + 471 WP_000136192.1 YlaC family protein -
BS647_RS24850 (4580647) 4580647..4582197 + 1551 WP_005098218.1 cyclic-guanylate-specific phosphodiesterase PdeB -
BS647_RS24855 (4582239) 4582239..4582592 - 354 WP_000878140.1 DUF1428 domain-containing protein -
BS647_RS24865 (4582971) 4582971..4583282 + 312 WP_000409911.1 MGMT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4575546..4576937 1391
flank IS/Tn - - 4583312..4584640 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T74327 WP_001291435.1 NZ_CP020342:4578956-4579174 [Shigella flexneri 1a]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T74327 NZ_CP020342:4578956-4579174 [Shigella flexneri 1a]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14513.38 Da        Isoelectric Point: 4.9091

>AT74327 WP_000344797.1 NZ_CP020342:4578556-4578930 [Shigella flexneri 1a]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQALQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT74327 NZ_CP020342:4578556-4578930 [Shigella flexneri 1a]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGTTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGCTCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAACGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TPD8

References