Detailed information of TA system TA074324

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3567487..3567712
• Replicon: chromosome
• Accession: NZ_CP020342
• Organism: Shigella flexneri 1a strain 0439

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: BS647_RS19270
• Protein ID: WP_000813254.1
• Coordinates: 3567487..3567642 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3567654..3567712 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BS647_RS19225	3562630..3563112	-	483	WP_000068107.1	ORF6N domain-containing protein	-
BS647_RS19230	3563183..3564322	-	1140	WP_000088354.1	IS3 family transposase	-
BS647_RS19235	3564384..3564557	-	174	WP_000504450.1	hypothetical protein	-
BS647_RS19240	3564710..3565264	-	555	WP_000640143.1	DUF1133 family protein	-
BS647_RS19245	3565261..3565551	-	291	WP_000228038.1	DUF1364 domain-containing protein	-
BS647_RS19250	3565551..3566150	-	600	WP_000940329.1	DUF1367 family protein	-
BS647_RS19260	3566284..3566981	+	698	WP_223368647.1	IS1 family transposase	-
BS647_RS19270	3567487..3567642	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3567654..3567712	+	59	-	-	Antitoxin
BS647_RS19280	3568097..3568462	-	366	WP_000610655.1	DUF551 domain-containing protein	-
BS647_RS19285	3568462..3569127	-	666	WP_000208062.1	hypothetical protein	-
BS647_RS19290	3569124..3569489	-	366	WP_001229297.1	HNH endonuclease signature motif containing protein	-
BS647_RS19295	3569491..3569709	-	219	WP_000256998.1	DUF4014 family protein	-
BS647_RS19300	3569802..3570158	-	357	WP_005048249.1	hypothetical protein	-
BS647_RS19305	3570216..3570638	-	423	WP_001118168.1	DUF977 family protein	-
BS647_RS19310	3570653..3571399	-	747	WP_000788996.1	ATP-binding protein	-
BS647_RS28515	3571545..3571714	-	170	Protein_3486	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	sitABCD	ipaH9.8	3516279..3580744	64465

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T74324 WP_000813254.1 NZ_CP020342:c3567642-3567487 [Shigella flexneri 1a]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T74324 NZ_CP020342:c3567642-3567487 [Shigella flexneri 1a]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT74324 NZ_CP020342:3567654-3567712 [Shigella flexneri 1a]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATTTGTGAGACATAGATTGGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin