Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 44752..45446 Replicon chromosome
Accession NZ_CP020342
Organism Shigella flexneri 1a strain 0439

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag BS647_RS00345 Protein ID WP_001263491.1
Coordinates 44752..45150 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag BS647_RS00350 Protein ID WP_000554759.1
Coordinates 45153..45446 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BS647_RS00315 (40091) 40091..40549 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
BS647_RS00320 (40811) 40811..42268 + 1458 WP_001293030.1 cytosol nonspecific dipeptidase -
BS647_RS00325 (42325) 42325..42885 - 561 Protein_57 peptide chain release factor H -
BS647_RS00330 (43001) 43001..44044 - 1044 WP_005053205.1 RNA ligase RtcB family protein -
BS647_RS00340 (44290) 44290..44742 - 453 WP_001059860.1 GNAT family N-acetyltransferase -
BS647_RS00345 (44752) 44752..45150 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
BS647_RS00350 (45153) 45153..45446 - 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
BS647_RS00355 (45498) 45498..46553 - 1056 WP_001226172.1 DNA polymerase IV -
BS647_RS00360 (46624) 46624..47409 - 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
BS647_RS00365 (47381) 47381..49093 + 1713 Protein_64 flagellar biosynthesis protein FlhA -
BS647_RS00370 (49310) 49310..49807 - 498 WP_000006251.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gtrB 2202..52366 50164


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T74308 WP_001263491.1 NZ_CP020342:c45150-44752 [Shigella flexneri 1a]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T74308 NZ_CP020342:c45150-44752 [Shigella flexneri 1a]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT74308 WP_000554759.1 NZ_CP020342:c45446-45153 [Shigella flexneri 1a]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT74308 NZ_CP020342:c45446-45153 [Shigella flexneri 1a]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
AGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTTCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
AAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References