Detailed information of TA system TA074305

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 4327195..4328039
• Replicon: chromosome
• Accession: NZ_CP020339
• Organism: Shigella flexneri 4c strain 0702

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: BS653_RS23405
• Protein ID: WP_014532172.1
• Coordinates: 4327195..4327656 (-)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: A5H0K0
• Locus tag: BS653_RS23415
• Protein ID: WP_005053053.1
• Coordinates: 4327728..4328039 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BS653_RS23390 (4322363)	4322363..4324093	-	1731	WP_000645013.1	hypothetical protein	-
BS653_RS23395 (4324146)	4324146..4326299	-	2154	WP_005060811.1	SEL1-like repeat protein	-
BS653_RS23400 (4326450)	4326450..4327147	+	698	WP_223368647.1	IS1 family transposase	-
BS653_RS23405 (4327195)	4327195..4327656	-	462	WP_014532172.1	GNAT family N-acetyltransferase	Toxin
BS653_RS23415 (4327728)	4327728..4328039	-	312	WP_005053053.1	DUF1778 domain-containing protein	Antitoxin
BS653_RS23420 (4328223)	4328223..4329113	-	891	WP_000971346.1	heme o synthase	-
BS653_RS23425 (4329125)	4329125..4329454	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
BS653_RS23430 (4329454)	4329454..4330068	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
BS653_RS23435 (4330058)	4330058..4332049	-	1992	Protein_4263	cytochrome o ubiquinol oxidase subunit I	-
BS653_RS23440 (4332071)	4332071..4332568	-	498	Protein_4264	COX aromatic rich motif-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP037923 Shigella flexneri 5a str. M90T 4168367..4168870 0.007	flank	IS/Tn	-	-	4326644..4327147	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (72-133)

Antitoxin

• Vibrio phage ICP1, Orf50 (15-97)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16623.52 Da
• Isoelectric Point: 9.9538

>T74305 WP_014532172.1 NZ_CP020339:c4327656-4327195 [Shigella flexneri 4c]
MIAVTLLSINLYALRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVMLGGAR
KYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFYARLGFVQLSATPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 462 bp

>T74305 NZ_CP020339:c4327656-4327195 [Shigella flexneri 4c]
TTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACTG
TGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAGAC
AACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGGAGCGCGG
AAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAAAAATTCACCCGGCATTACC
AATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCGA
CACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11394.07 Da
• Isoelectric Point: 10.3388

>AT74305 WP_005053053.1 NZ_CP020339:c4328039-4327728 [Shigella flexneri 4c]
MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT74305 NZ_CP020339:c4328039-4327728 [Shigella flexneri 4c]
ATGCCTGGAAAAACAGCCGCTCTATACGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A5H0K0