Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4228434..4229128 Replicon chromosome
Accession NZ_CP020339
Organism Shigella flexneri 4c strain 0702

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag BS653_RS22795 Protein ID WP_001263491.1
Coordinates 4228730..4229128 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag BS653_RS22790 Protein ID WP_000554759.1
Coordinates 4228434..4228727 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BS653_RS22770 (4224073) 4224073..4224570 + 498 WP_000006251.1 REP-associated tyrosine transposase RayT -
BS653_RS22775 (4224787) 4224787..4226499 - 1713 Protein_4136 flagellar biosynthesis protein FlhA -
BS653_RS22780 (4226471) 4226471..4227256 + 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
BS653_RS22785 (4227327) 4227327..4228382 + 1056 WP_001226172.1 DNA polymerase IV -
BS653_RS22790 (4228434) 4228434..4228727 + 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
BS653_RS22795 (4228730) 4228730..4229128 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
BS653_RS22800 (4229138) 4229138..4229590 + 453 WP_001059860.1 GNAT family N-acetyltransferase -
BS653_RS22810 (4229836) 4229836..4230879 + 1044 WP_005053205.1 RNA ligase RtcB family protein -
BS653_RS22815 (4230995) 4230995..4231555 + 561 Protein_4143 peptide chain release factor H -
BS653_RS22820 (4231612) 4231612..4233069 - 1458 WP_001293030.1 cytosol nonspecific dipeptidase -
BS653_RS22825 (4233331) 4233331..4233789 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T74304 WP_001263491.1 NZ_CP020339:4228730-4229128 [Shigella flexneri 4c]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T74304 NZ_CP020339:4228730-4229128 [Shigella flexneri 4c]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT74304 WP_000554759.1 NZ_CP020339:4228434-4228727 [Shigella flexneri 4c]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT74304 NZ_CP020339:4228434-4228727 [Shigella flexneri 4c]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
AGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTTCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
AAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References