Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 1393876..1394101 Replicon chromosome
Accession NZ_CP020339
Organism Shigella flexneri 4c strain 0702

Toxin (Protein)


Gene name hokW Uniprot ID S1ELB5
Locus tag BS653_RS08025 Protein ID WP_000813254.1
Coordinates 1393876..1394031 (-) Length 52 a.a.

Antitoxin (RNA)


Gene name sokW
Locus tag -
Coordinates 1394043..1394101 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BS653_RS07960 1389188..1389538 - 351 WP_005049241.1 IS66 family insertion sequence element accessory protein TnpB -
BS653_RS07965 1389535..1390209 - 675 WP_004967157.1 IS66-like element accessory protein TnpA -
BS653_RS07970 1390308..1390523 - 216 WP_000839572.1 class II holin family protein -
BS653_RS08000 1391319..1392007 - 689 Protein_1459 bacteriophage antitermination protein Q -
BS653_RS08005 1392004..1392369 - 366 WP_000140020.1 RusA family crossover junction endodeoxyribonuclease -
BS653_RS08010 1392370..1393428 - 1059 WP_001265248.1 DUF968 domain-containing protein -
BS653_RS08015 1393430..1393708 - 279 WP_011069426.1 hypothetical protein -
BS653_RS08025 1393876..1394031 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD Toxin
- 1394043..1394101 + 59 - - Antitoxin
BS653_RS08040 1394683..1395099 - 417 WP_005069274.1 hypothetical protein -
BS653_RS08045 1395126..1395266 + 141 Protein_1465 DUF4224 domain-containing protein -
BS653_RS08050 1395266..1396316 + 1051 Protein_1466 tyrosine-type recombinase/integrase -
BS653_RS08060 1396527..1397324 + 798 WP_001325918.1 DgsA anti-repressor MtfA -
BS653_RS08070 1397662..1398924 + 1263 Protein_1468 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - ipaH9.8 1362027..1421087 59060


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(6-47)


Sequences

Toxin        


Download         Length: 52 a.a.        Molecular weight: 5736.89 Da        Isoelectric Point: 6.1531

>T74298 WP_000813254.1 NZ_CP020339:c1394031-1393876 [Shigella flexneri 4c]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Download         Length: 156 bp

>T74298 NZ_CP020339:c1394031-1393876 [Shigella flexneri 4c]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTTATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin


Download         Length: 59 bp

>AT74298 NZ_CP020339:1394043-1394101 [Shigella flexneri 4c]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TMW0

Antitoxin

Download structure file

References