Detailed information of TA system TA074287

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 726003..726228
• Replicon: chromosome
• Accession: NZ_CP020339
• Organism: Shigella flexneri 4c strain 0702

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: BS653_RS04045
• Protein ID: WP_000813254.1
• Coordinates: 726073..726228 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	726003..726061 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BS653_RS27360	722001..722170	+	170	Protein_739	hypothetical protein	-
BS653_RS04005	722316..723062	+	747	WP_000788996.1	ATP-binding protein	-
BS653_RS04010	723077..723499	+	423	WP_001118168.1	DUF977 family protein	-
BS653_RS04015	723557..723913	+	357	WP_005048249.1	hypothetical protein	-
BS653_RS04020	724006..724224	+	219	WP_000256998.1	DUF4014 family protein	-
BS653_RS04025	724226..724591	+	366	WP_001229297.1	HNH endonuclease signature motif containing protein	-
BS653_RS04030	724588..725253	+	666	WP_000208062.1	hypothetical protein	-
BS653_RS04035	725253..725618	+	366	WP_000610655.1	DUF551 domain-containing protein	-
-	726003..726061	-	59	-	-	Antitoxin
BS653_RS04045	726073..726228	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
BS653_RS04065	727565..728164	+	600	WP_000940329.1	DUF1367 family protein	-
BS653_RS04070	728164..728454	+	291	WP_000228038.1	DUF1364 domain-containing protein	-
BS653_RS04075	728451..729005	+	555	WP_000640143.1	DUF1133 family protein	-
BS653_RS04080	729158..729331	+	174	WP_000504450.1	hypothetical protein	-
BS653_RS04085	729393..730532	+	1140	WP_000088353.1	IS3 family transposase	-
BS653_RS04090	730603..731085	+	483	WP_000068107.1	ORF6N domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	sitABCD	ipaH9.8	712971..767148	54177

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T74287 WP_000813254.1 NZ_CP020339:726073-726228 [Shigella flexneri 4c]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T74287 NZ_CP020339:726073-726228 [Shigella flexneri 4c]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT74287 NZ_CP020339:c726061-726003 [Shigella flexneri 4c]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATTTGTGAGACATAGATTGGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin