Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 2222506..2222731 Replicon chromosome
Accession NZ_CP020336
Organism Shigella flexneri 4c strain 1602

Toxin (Protein)


Gene name hokW Uniprot ID S1ELB5
Locus tag BS654_RS12515 Protein ID WP_000813254.1
Coordinates 2222506..2222661 (-) Length 52 a.a.

Antitoxin (RNA)


Gene name sokW
Locus tag -
Coordinates 2222673..2222731 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BS654_RS12450 2217818..2218168 - 351 WP_005049241.1 IS66 family insertion sequence element accessory protein TnpB -
BS654_RS12455 2218165..2218839 - 675 WP_004967157.1 IS66-like element accessory protein TnpA -
BS654_RS12460 2218938..2219153 - 216 WP_000839572.1 class II holin family protein -
BS654_RS12490 2219949..2220637 - 689 Protein_2281 bacteriophage antitermination protein Q -
BS654_RS12495 2220634..2220999 - 366 WP_000140020.1 RusA family crossover junction endodeoxyribonuclease -
BS654_RS12500 2221000..2222058 - 1059 WP_001265248.1 DUF968 domain-containing protein -
BS654_RS12505 2222060..2222338 - 279 WP_011069426.1 hypothetical protein -
BS654_RS12515 2222506..2222661 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD Toxin
- 2222673..2222731 + 59 - - Antitoxin
BS654_RS12530 2223313..2223729 - 417 WP_005069274.1 hypothetical protein -
BS654_RS12535 2223756..2223896 + 141 Protein_2287 DUF4224 domain-containing protein -
BS654_RS12540 2223896..2224946 + 1051 Protein_2288 tyrosine-type recombinase/integrase -
BS654_RS12550 2225157..2225954 + 798 WP_001325918.1 DgsA anti-repressor MtfA -
BS654_RS12560 2226292..2227554 + 1263 Protein_2290 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - ipaH9.8 2205106..2249718 44612


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(6-47)


Sequences

Toxin        


Download         Length: 52 a.a.        Molecular weight: 5736.89 Da        Isoelectric Point: 6.1531

>T74275 WP_000813254.1 NZ_CP020336:c2222661-2222506 [Shigella flexneri 4c]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Download         Length: 156 bp

>T74275 NZ_CP020336:c2222661-2222506 [Shigella flexneri 4c]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTTATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin


Download         Length: 59 bp

>AT74275 NZ_CP020336:2222673-2222731 [Shigella flexneri 4c]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TMW0

Antitoxin

Download structure file

References