Detailed information of TA system TA074264

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1548828..1549053
• Replicon: chromosome
• Accession: NZ_CP020336
• Organism: Shigella flexneri 4c strain 1602

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: BS654_RS08485
• Protein ID: WP_000813254.1
• Coordinates: 1548898..1549053 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1548828..1548886 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BS654_RS28890	1544826..1544995	+	170	Protein_1555	hypothetical protein	-
BS654_RS08445	1545141..1545887	+	747	WP_094085497.1	ATP-binding protein	-
BS654_RS08450	1545902..1546324	+	423	WP_001118168.1	DUF977 family protein	-
BS654_RS08455	1546382..1546738	+	357	WP_005048249.1	hypothetical protein	-
BS654_RS08460	1546831..1547049	+	219	WP_000256998.1	DUF4014 family protein	-
BS654_RS08465	1547051..1547416	+	366	Protein_1560	HNH endonuclease	-
BS654_RS08470	1547413..1548078	+	666	WP_000208062.1	hypothetical protein	-
BS654_RS08475	1548078..1548443	+	366	WP_000610655.1	DUF551 domain-containing protein	-
-	1548828..1548886	-	59	-	-	Antitoxin
BS654_RS08485	1548898..1549053	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
BS654_RS08505	1550390..1550989	+	600	WP_000940329.1	DUF1367 family protein	-
BS654_RS08510	1550989..1551279	+	291	WP_000228038.1	DUF1364 domain-containing protein	-
BS654_RS08515	1551276..1551830	+	555	WP_000640143.1	DUF1133 family protein	-
BS654_RS08520	1551983..1552156	+	174	WP_000504450.1	hypothetical protein	-
BS654_RS08525	1552218..1553357	+	1140	WP_000088353.1	IS3 family transposase	-
BS654_RS08530	1553428..1553910	+	483	WP_000068107.1	ORF6N domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	sitABCD	ipaH9.8	1540911..1605036	64125

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T74264 WP_000813254.1 NZ_CP020336:1548898-1549053 [Shigella flexneri 4c]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T74264 NZ_CP020336:1548898-1549053 [Shigella flexneri 4c]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT74264 NZ_CP020336:c1548886-1548828 [Shigella flexneri 4c]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATTTGTGAGACATAGATTGGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin