Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 397449..398143 Replicon chromosome
Accession NZ_CP020336
Organism Shigella flexneri 4c strain 1602

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag BS654_RS02070 Protein ID WP_001263491.1
Coordinates 397745..398143 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag BS654_RS02065 Protein ID WP_000554759.1
Coordinates 397449..397742 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BS654_RS02045 (393088) 393088..393585 + 498 WP_000006251.1 REP-associated tyrosine transposase RayT -
BS654_RS02050 (393802) 393802..395514 - 1713 Protein_386 flagellar biosynthesis protein FlhA -
BS654_RS02055 (395486) 395486..396271 + 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
BS654_RS02060 (396342) 396342..397397 + 1056 WP_001226172.1 DNA polymerase IV -
BS654_RS02065 (397449) 397449..397742 + 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
BS654_RS02070 (397745) 397745..398143 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
BS654_RS02075 (398153) 398153..398605 + 453 WP_001059860.1 GNAT family N-acetyltransferase -
BS654_RS02085 (398851) 398851..399894 + 1044 WP_005053205.1 RNA ligase RtcB family protein -
BS654_RS02090 (400010) 400010..400570 + 561 Protein_393 peptide chain release factor H -
BS654_RS02095 (400627) 400627..402084 - 1458 WP_001293030.1 cytosol nonspecific dipeptidase -
BS654_RS02100 (402346) 402346..402804 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T74260 WP_001263491.1 NZ_CP020336:397745-398143 [Shigella flexneri 4c]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T74260 NZ_CP020336:397745-398143 [Shigella flexneri 4c]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT74260 WP_000554759.1 NZ_CP020336:397449-397742 [Shigella flexneri 4c]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT74260 NZ_CP020336:397449-397742 [Shigella flexneri 4c]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
AGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTTCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
AAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References