Detailed information of TA system TA074259

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 5306130..5306778
• Replicon: chromosome
• Accession: NZ_CP020335
• Organism: Photorhabdus akhurstii strain 0813-124 phase II

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: B0X70_RS22745
• Protein ID: WP_217470457.1
• Coordinates: 5306416..5306778 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1B8YBU2
• Locus tag: B0X70_RS22740
• Protein ID: WP_049585084.1
• Coordinates: 5306130..5306429 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0X70_RS22715 (B0X70_22895)	5301148..5301414	+	267	WP_011148622.1	helix-turn-helix transcriptional regulator	-
B0X70_RS22720 (B0X70_22900)	5301516..5302454	-	939	WP_040151153.1	malate dehydrogenase	-
B0X70_RS22725 (B0X70_22905)	5302912..5303382	+	471	WP_036776550.1	transcriptional regulator ArgR	-
B0X70_RS22730 (B0X70_22910)	5303428..5304798	-	1371	WP_217470456.1	UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase	-
B0X70_RS22735 (B0X70_22915)	5304964..5305968	+	1005	WP_046396595.1	class 1 fructose-bisphosphatase	-
B0X70_RS22740 (B0X70_22920)	5306130..5306429	-	300	WP_049585084.1	helix-turn-helix transcriptional regulator	Antitoxin
B0X70_RS22745 (B0X70_22925)	5306416..5306778	-	363	WP_217470457.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B0X70_RS22750 (B0X70_22930)	5307111..5307641	+	531	WP_036776561.1	inorganic diphosphatase	-
B0X70_RS22755 (B0X70_22935)	5307742..5308089	-	348	WP_065392172.1	gamma-glutamylcyclotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13813.92 Da
• Isoelectric Point: 8.4861

>T74259 WP_217470457.1 NZ_CP020335:c5306778-5306416 [Photorhabdus akhurstii]
VWIIILTRRFEDWFDEQDISTQKKILADLSVLEVFGPQLGRPVVDSLYGSKFNNMKELRIQCKGRPIRAFFAFDPLRRAI
VLCAGDKTGNEKRFYKEMISVADAEYKAHLASLEGKNENS

Nucleotide

• Length: 363 bp

>T74259 NZ_CP020335:c5306778-5306416 [Photorhabdus akhurstii]
GTGTGGATTATAATTTTAACTCGTCGATTTGAAGATTGGTTTGATGAGCAAGATATCAGTACCCAGAAAAAAATATTGGC
AGACCTTAGTGTGTTAGAAGTGTTTGGTCCTCAACTAGGCCGTCCTGTTGTTGACTCTTTGTATGGTTCAAAATTTAACA
ATATGAAAGAGTTAAGAATTCAGTGTAAGGGGAGGCCAATAAGAGCATTTTTTGCCTTTGACCCATTAAGACGAGCTATT
GTTCTATGTGCTGGTGATAAGACAGGGAACGAAAAGCGTTTTTACAAAGAAATGATCTCGGTGGCTGATGCTGAGTATAA
AGCTCACTTAGCGAGTTTGGAGGGTAAAAATGAAAACTCTTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11172.99 Da
• Isoelectric Point: 5.8882

>AT74259 WP_049585084.1 NZ_CP020335:c5306429-5306130 [Photorhabdus akhurstii]
MKTLKDKIREFSVEDRADIKQMADEMILETGLQLMREELNISQKKLADSLGISQPAITQIEQRGNDIKLATLKRYVEAMG
GKLSLTVELPTGDGRIFHI

Nucleotide

• Length: 300 bp

>AT74259 NZ_CP020335:c5306429-5306130 [Photorhabdus akhurstii]
ATGAAAACTCTTAAAGATAAGATACGAGAATTTTCTGTTGAAGATCGAGCAGATATAAAACAGATGGCTGATGAAATGAT
TTTAGAAACTGGTTTGCAACTGATGAGAGAAGAGCTGAATATCTCTCAGAAAAAACTGGCGGATAGTTTAGGTATTAGTC
AGCCTGCAATTACGCAAATAGAACAACGGGGTAACGATATAAAACTGGCTACGTTAAAGCGTTATGTTGAAGCTATGGGA
GGCAAACTTAGCCTAACCGTAGAACTCCCAACTGGGGATGGGAGAATTTTTCATATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1B8YBU2