Detailed information of TA system TA074243

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 2466420..2467070
• Replicon: chromosome
• Accession: NZ_CP020335
• Organism: Photorhabdus akhurstii strain 0813-124 phase II

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: B0X70_RS10795
• Protein ID: WP_105395394.1
• Coordinates: 2466735..2467070 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: B0X70_RS10790
• Protein ID: WP_105395393.1
• Coordinates: 2466420..2466734 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0X70_RS10770 (B0X70_10875)	2462066..2462812	+	747	WP_217471486.1	hypothetical protein	-
B0X70_RS10775 (B0X70_10880)	2462904..2464883	+	1980	WP_217471487.1	type VI secretion system tip protein TssI/VgrG	-
B0X70_RS10780 (B0X70_10885)	2464880..2465659	+	780	WP_217471488.1	DUF4123 domain-containing protein	-
B0X70_RS10785 (B0X70_10890)	2465841..2466404	+	564	WP_217471489.1	VOC family protein	-
B0X70_RS10790 (B0X70_10895)	2466420..2466734	-	315	WP_105395393.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
B0X70_RS10795 (B0X70_10900)	2466735..2467070	-	336	WP_105395394.1	toxin	Toxin
B0X70_RS10800 (B0X70_10905)	2467141..2467251	+	111	Protein_2080	VOC family protein	-
B0X70_RS10805 (B0X70_10910)	2467419..2468165	+	747	WP_046395062.1	copper homeostasis protein CutC	-
B0X70_RS10810 (B0X70_10915)	2468206..2469177	-	972	WP_217471490.1	tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB	-
B0X70_RS10815 (B0X70_10920)	2469174..2469932	-	759	WP_046395065.1	carboxy-S-adenosyl-L-methionine synthase CmoA	-
B0X70_RS10820 (B0X70_10925)	2470211..2470462	+	252	WP_036806437.1	plasmid stabilization protein	-
B0X70_RS10825 (B0X70_10930)	2470452..2470733	+	282	WP_217471491.1	type II toxin-antitoxin system RelE/ParE family toxin	-
B0X70_RS10830 (B0X70_10935)	2470863..2471258	-	396	WP_036775538.1	MAPEG family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Antitoxin MqsA/HigA-2 (12-98)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 13349.40 Da
• Isoelectric Point: 10.1960

>T74243 WP_105395394.1 NZ_CP020335:c2467070-2466735 [Photorhabdus akhurstii]
MEAVFIELPPFERYRHNYLSDEEFRSFQNILLKNPTAGDVIQHTGGLRKVRFTDSRRKKGKRSGIRVIYYWWTSKSQFML
FTIYDKDEMSDLTKEQCESLRKMLEMRKKGA

Nucleotide

• Length: 336 bp

>T74243 NZ_CP020335:c2467070-2466735 [Photorhabdus akhurstii]
ATGGAAGCTGTTTTTATTGAACTACCACCTTTTGAACGCTACCGCCATAACTACCTGAGTGATGAGGAGTTTCGATCCTT
CCAAAATATACTGCTGAAAAATCCAACAGCAGGGGACGTCATCCAACATACCGGCGGATTACGTAAAGTGCGTTTTACAG
ATTCCCGAAGAAAAAAGGGAAAACGGAGCGGTATTCGGGTGATTTACTATTGGTGGACATCTAAATCGCAATTCATGCTT
TTCACTATCTACGATAAAGATGAGATGTCAGACTTAACTAAAGAACAATGCGAATCCTTGCGTAAGATGTTAGAAATGAG
AAAAAAAGGAGCATAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11977.74 Da
• Isoelectric Point: 10.1690

>AT74243 WP_105395393.1 NZ_CP020335:c2466734-2466420 [Photorhabdus akhurstii]
MNRDLFAELNEGMKAWEEANESKLTLKTHKRRLKNDISINPDELKSIRSKLNISQAVFAQYLHTGETTYQNWEQGRAKPN
KQAVLLIKMVARHPQALEFLAGLT

Nucleotide

• Length: 315 bp

>AT74243 NZ_CP020335:c2466734-2466420 [Photorhabdus akhurstii]
ATGAACCGTGATCTATTTGCAGAACTCAATGAAGGAATGAAAGCTTGGGAAGAGGCTAATGAAAGCAAACTCACATTGAA
AACGCATAAGAGGAGGTTAAAAAACGATATCTCCATCAATCCAGATGAGTTGAAATCCATACGGAGTAAACTGAATATTT
CTCAAGCTGTTTTTGCCCAGTACCTGCACACCGGTGAAACGACATACCAGAACTGGGAACAAGGGCGGGCTAAACCGAAT
AAACAAGCTGTATTACTGATTAAAATGGTGGCACGCCACCCGCAGGCACTAGAGTTTCTTGCCGGCCTCACATAG