Detailed information of TA system TA074241

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2143051..2143678
• Replicon: chromosome
• Accession: NZ_CP020335
• Organism: Photorhabdus akhurstii strain 0813-124 phase II

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1B8YGH7
• Locus tag: B0X70_RS09330
• Protein ID: WP_046394394.1
• Coordinates: 2143051..2143338 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: B0X70_RS09335
• Protein ID: WP_046394395.1
• Coordinates: 2143355..2143678 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0X70_RS09305 (B0X70_09415)	2138524..2140035	-	1512	WP_110090317.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-
B0X70_RS09310 (B0X70_09420)	2140339..2141172	+	834	WP_036775810.1	MurR/RpiR family transcriptional regulator	-
B0X70_RS09315 (B0X70_09425)	2141383..2141739	+	357	WP_214038928.1	hypothetical protein	-
B0X70_RS09320 (B0X70_09430)	2141884..2142219	+	336	WP_040152347.1	tRNA-binding protein	-
B0X70_RS09325 (B0X70_09435)	2142276..2142983	+	708	WP_214038929.1	AraC family transcriptional regulator	-
B0X70_RS09330 (B0X70_09440)	2143051..2143338	+	288	WP_046394394.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B0X70_RS09335 (B0X70_09445)	2143355..2143678	+	324	WP_046394395.1	HigA family addiction module antitoxin	Antitoxin
B0X70_RS09340 (B0X70_09450)	2143744..2145282	-	1539	WP_214038930.1	peptide MFS transporter	-
B0X70_RS09345 (B0X70_09455)	2146007..2146948	-	942	WP_217471369.1	Kdo(2)-lipid IV(A) acyltransferase	-
B0X70_RS09350 (B0X70_09460)	2147232..2147468	+	237	WP_036807294.1	DinI family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (5-85)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11433.11 Da
• Isoelectric Point: 7.8969

>T74241 WP_046394394.1 NZ_CP020335:2143051-2143338 [Photorhabdus akhurstii]
MAVEFKDRWLERFYEQDLPHKCIPASLQRALYRKLQILDASVKEADLRIPPGNHFEHLNGYLRGWCSIRVNRQYRLIFQW
IDGIAVDTYLDPHNY

Nucleotide

• Length: 288 bp

>T74241 NZ_CP020335:2143051-2143338 [Photorhabdus akhurstii]
ATGGCTGTTGAATTCAAGGATAGATGGCTAGAAAGATTTTACGAACAGGATTTACCGCACAAATGCATACCTGCATCTTT
ACAGCGTGCGTTATACAGAAAACTACAAATTCTGGATGCATCGGTTAAAGAAGCTGATTTAAGAATCCCTCCGGGAAACC
ATTTTGAACATTTAAATGGTTATTTAAGGGGATGGTGCTCAATAAGAGTAAACAGGCAATACAGGCTTATTTTTCAATGG
ATTGATGGTATTGCAGTTGATACATATCTGGATCCTCATAACTATTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11904.88 Da
• Isoelectric Point: 9.9254

>AT74241 WP_046394395.1 NZ_CP020335:2143355-2143678 [Photorhabdus akhurstii]
MRNTKRRPATVGEILVSEFLAPLNIEINMLAEAMGVHRNTLSRIVHDKGSLTVPMAIKLAAALGNTAEFWLNIQHAVELW
DVRNHAYQAESLNVKQIVKPSIITAVH

Nucleotide

• Length: 324 bp

>AT74241 NZ_CP020335:2143355-2143678 [Photorhabdus akhurstii]
ATGAGAAATACAAAACGTCGCCCTGCGACAGTGGGTGAAATATTGGTGAGTGAGTTTCTGGCGCCTTTGAATATTGAAAT
TAATATGCTTGCGGAAGCTATGGGTGTTCACCGTAATACATTAAGTAGGATTGTGCATGATAAAGGTTCGCTGACCGTTC
CTATGGCGATCAAATTGGCAGCGGCTTTGGGGAACACCGCAGAGTTTTGGCTGAATATTCAGCATGCCGTTGAACTATGG
GATGTGCGTAATCATGCATATCAAGCGGAGTCATTGAATGTGAAACAGATAGTTAAGCCATCAATCATAACGGCAGTGCA
TTGA

Structures

Toxin

• AlphaFold DB: A0A1B8YGH7