Detailed information of TA system TA074229

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4734462..4735051
• Replicon: chromosome
• Accession: NZ_CP020334
• Organism: Xanthomonas oryzae pv. oryzae strain XM9

Toxin (Protein)

• Gene name: graT
• Uniprot ID: G7TIR7
• Locus tag: B4599_RS23465
• Protein ID: WP_012443754.1
• Coordinates: 4734770..4735051 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: B4599_RS23460
• Protein ID: WP_011260746.1
• Coordinates: 4734462..4734752 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B4599_RS23420	4729615..4730223	-	609	WP_113195694.1	DUF4123 domain-containing protein	-
B4599_RS23425	4730226..4730660	-	435	WP_011409747.1	PAAR domain-containing protein	-
B4599_RS23430	4730776..4731021	-	246	WP_069960049.1	hypothetical protein	-
B4599_RS23440	4731357..4731689	+	333	WP_011260743.1	hypothetical protein	-
B4599_RS23450	4732139..4733533	-	1395	WP_027703652.1	nucleoside hydrolase	-
B4599_RS23460	4734462..4734752	-	291	WP_011260746.1	HigA family addiction module antidote protein	Antitoxin
B4599_RS23465	4734770..4735051	-	282	WP_012443754.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B4599_RS23470	4735146..4737398	-	2253	WP_069960050.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11066.83 Da
• Isoelectric Point: 10.5318

>T74229 WP_012443754.1 NZ_CP020334:c4735051-4734770 [Xanthomonas oryzae pv. oryzae]
VIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQRRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T74229 NZ_CP020334:c4735051-4734770 [Xanthomonas oryzae pv. oryzae]
GTGATCAGGAGCTTTATCGACAAGGACGCCGAAAAGATCTGGCTGGGTGAGCGTTCCCGCCGGTTGCCTGCCGACATCCA
ATTGGTCGCGCGCCGCAAGTTGCGCATGCTCAATGCCGCCGCACATCTCGACGATCTGCGGATCCCGCCCGCCAACCGGC
TGGAAGCGTTGAAGGGCCAGCGGCGCGGCCAGTACAGCATCCGGATCAACGATCAATGGCGCATCTGCTTCCGATGGATG
GAAGGCGATGTGGTCCAGGTCGAAATCGTCGATTACCACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT74229 WP_011260746.1 NZ_CP020334:c4734752-4734462 [Xanthomonas oryzae pv. oryzae]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT74229 NZ_CP020334:c4734752-4734462 [Xanthomonas oryzae pv. oryzae]
ATGACGGTCCTACCCAATATCCATCCTGGCGACATTCTGCTTGAGGAGTTTCTCGAGCCTCTGGGCATCAGCCAGAATGC
ACTGGCACGCGCCACCGGCGTCCCGCCGCGCCGTATCAACGAAATCGTGCTGGGCAAGCGCGGCATCACTGCCGACACTG
CCGTGCGTCTGGCCGCCGCGTTGGGCACCTCCGAGCGCTTCTGGCTGGGCCTGCAGGCCGACTACGAGCTGGAACAGGCG
CATCGCGCGCTGGGCGATTTGCCGGCACGGATCAAGCGGCTGGCGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A0K0GFW5