Detailed information of TA system TA074222

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 504590..505184
• Replicon: plasmid pMM593
• Accession: NZ_CP020331
• Organism: Martelella mediterranea DSM 17316 strain MACL11

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: Mame_RS24090
• Protein ID: WP_026173357.1
• Coordinates: 504903..505184 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: Mame_RS24085
• Protein ID: WP_018064118.1
• Coordinates: 504590..504886 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Mame_RS24065	500100..501086	+	987	WP_018064114.1	hypothetical protein	-
Mame_RS24070	501121..501615	+	495	WP_018064115.1	tripartite tricarboxylate transporter TctB family protein	-
Mame_RS24075	501619..503136	+	1518	WP_018064116.1	tripartite tricarboxylate transporter permease	-
Mame_RS24080	503378..504499	+	1122	WP_018064117.1	Gfo/Idh/MocA family oxidoreductase	-
Mame_RS24085	504590..504886	-	297	WP_018064118.1	HigA family addiction module antidote protein	Antitoxin
Mame_RS24090	504903..505184	-	282	WP_026173357.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Mame_RS24095	505322..505525	-	204	WP_018064120.1	hypothetical protein	-
Mame_RS24100	505530..506423	-	894	WP_018064121.1	LysR family transcriptional regulator	-
Mame_RS24105	506525..507337	+	813	WP_018064122.1	SDR family NAD(P)-dependent oxidoreductase	-
Mame_RS24110	507469..507912	-	444	WP_018064123.1	helix-turn-helix transcriptional regulator	-
Mame_RS24115	508030..508701	+	672	WP_155122273.1	NmrA/HSCARG family protein	-
Mame_RS24120	508774..509722	+	949	WP_155122056.1	IS630 family transposase	-
Mame_RS24125	509729..509896	-	168	WP_155122274.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..593079	593079

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10461.86 Da
• Isoelectric Point: 8.9788

>T74222 WP_026173357.1 NZ_CP020331:c505184-504903 [Martelella mediterranea DSM 17316]
MIRSYRNATTESVASGKAPKGFPADIMRAAIRKLTMIDNAHALDDLRSPPGNRLEALKGNRKGQYSIRINDQWRICFVWT
DGGAEDVEIVDYH

Nucleotide

• Length: 282 bp

>T74222 NZ_CP020331:c505184-504903 [Martelella mediterranea DSM 17316]
CTGATCCGATCCTATCGCAATGCCACGACGGAGAGTGTCGCCAGCGGCAAGGCGCCGAAAGGCTTTCCTGCCGATATAAT
GCGCGCGGCGATCCGAAAGCTCACGATGATTGACAATGCGCACGCCCTGGATGATTTGCGCTCGCCGCCGGGCAACAGGC
TGGAGGCGTTGAAGGGCAATAGAAAAGGTCAGTACTCGATCAGGATCAACGATCAGTGGCGCATCTGCTTTGTCTGGACG
GATGGCGGCGCGGAAGATGTCGAAATCGTCGATTACCATTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11087.86 Da
• Isoelectric Point: 6.4722

>AT74222 WP_018064118.1 NZ_CP020331:c504886-504590 [Martelella mediterranea DSM 17316]
MASILPPVHPGEILREEYLIPLNMSAGALAKRLHVPRTRIERIAKEETAITPDTALRLAKFFRTTPQFWLNMQDSFDLKR
QAEALKGEIDAIEAFEAA

Nucleotide

• Length: 297 bp

>AT74222 NZ_CP020331:c504886-504590 [Martelella mediterranea DSM 17316]
ATGGCCAGCATTCTGCCGCCGGTCCACCCGGGTGAAATCCTGCGGGAAGAATACCTGATCCCGCTGAACATGAGCGCCGG
CGCACTGGCGAAGCGCCTTCACGTGCCGCGCACCCGCATCGAACGGATCGCAAAGGAGGAAACGGCGATCACGCCGGATA
CGGCGCTGCGGCTCGCCAAATTCTTCCGCACCACGCCGCAATTCTGGCTGAACATGCAGGACAGCTTCGATCTGAAGCGC
CAGGCGGAAGCGCTGAAGGGCGAGATCGACGCGATCGAGGCATTCGAAGCGGCGTGA