Detailed information of TA system TA074205

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 8319..8561
• Replicon: plasmid unitig_5
• Accession: NZ_CP020117
• Organism: Escherichia coli strain AR_0104

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: AM483_RS25845
• Protein ID: WP_001312861.1
• Coordinates: 8403..8561 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	8319..8359 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM483_RS25820	3514..5472	+	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-
AM483_RS25825	5527..5961	+	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
AM483_RS25830	5958..6677	+	720	WP_001276232.1	plasmid SOS inhibition protein A	-
-	6689..6875	+	187	NuclAT_0	-	-
-	6689..6875	+	187	NuclAT_0	-	-
-	6689..6875	+	187	NuclAT_0	-	-
-	6689..6875	+	187	NuclAT_0	-	-
AM483_RS25840	6923..8292	+	1370	WP_085947770.1	IS3-like element IS150 family transposase	-
-	8319..8359	+	41	NuclAT_1	-	Antitoxin
-	8319..8359	+	41	NuclAT_1	-	Antitoxin
-	8319..8359	+	41	NuclAT_1	-	Antitoxin
-	8319..8359	+	41	NuclAT_1	-	Antitoxin
AM483_RS25845	8403..8561	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
AM483_RS28530	9252..9458	+	207	WP_000275859.1	hypothetical protein	-
AM483_RS25870	9483..9770	+	288	WP_000107535.1	hypothetical protein	-
AM483_RS25875	9889..10710	+	822	WP_001234469.1	DUF945 domain-containing protein	-
AM483_RS25880	11007..11609	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
AM483_RS25885	11940..12323	+	384	WP_001151566.1	relaxosome protein TraM	-
AM483_RS25890	12457..13134	+	678	WP_001348626.1	PAS domain-containing protein	-
AM483_RS25895	13222..13443	+	222	WP_072277695.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5	senB	1..107210	107210

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T74205 WP_001312861.1 NZ_CP020117:8403-8561 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T74205 NZ_CP020117:8403-8561 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 41 bp

>AT74205 NZ_CP020117:8319-8359 [Escherichia coli]
TTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin