Detailed information of TA system TA074203

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4480461..4481063
• Replicon: chromosome
• Accession: NZ_CP020116
• Organism: Escherichia coli strain AR_0104

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: AM483_RS23290
• Protein ID: WP_000897302.1
• Coordinates: 4480752..4481063 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM483_RS23285
• Protein ID: WP_000356397.1
• Coordinates: 4480461..4480751 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM483_RS23245	4475768..4476697	+	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-
AM483_RS23250	4476879..4477121	-	243	WP_001068514.1	ribbon-helix-helix domain-containing protein	-
AM483_RS23255	4477411..4478259	+	849	WP_001038650.1	hypothetical protein	-
AM483_RS23260	4478575..4479024	+	450	WP_032140890.1	hypothetical protein	-
AM483_RS23265	4479209..4479427	-	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
AM483_RS23275	4479824..4480102	-	279	WP_001296612.1	hypothetical protein	-
AM483_RS23285	4480461..4480751	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
AM483_RS23290	4480752..4481063	-	312	WP_000897302.1	hypothetical protein	Toxin
AM483_RS23295	4481292..4482200	+	909	WP_001385591.1	alpha/beta hydrolase	-
AM483_RS23300	4482264..4483205	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AM483_RS23305	4483250..4483687	-	438	WP_000560981.1	D-tyrosyl-tRNA(Tyr) deacylase	-
AM483_RS23310	4483684..4484556	-	873	WP_000920762.1	virulence factor BrkB family protein	-
AM483_RS23315	4484550..4485149	-	600	WP_001296610.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T74203 WP_000897302.1 NZ_CP020116:c4481063-4480752 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T74203 NZ_CP020116:c4481063-4480752 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT74203 WP_000356397.1 NZ_CP020116:c4480751-4480461 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT74203 NZ_CP020116:c4480751-4480461 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416