Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3289115..3289794 Replicon chromosome
Accession NZ_CP020116
Organism Escherichia coli strain AR_0104

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag AM483_RS17230 Protein ID WP_000057523.1
Coordinates 3289492..3289794 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag AM483_RS17225 Protein ID WP_000806442.1
Coordinates 3289115..3289456 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM483_RS17215 3285359..3286291 - 933 WP_000883041.1 glutaminase A -
AM483_RS17220 3286553..3289057 + 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
AM483_RS17225 3289115..3289456 - 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
AM483_RS17230 3289492..3289794 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AM483_RS17235 3289927..3290721 + 795 WP_000365147.1 TraB/GumN family protein -
AM483_RS17245 3290925..3291233 + 309 Protein_3181 Cys-tRNA(Pro) deacylase -
AM483_RS17250 3291289..3291986 + 698 Protein_3182 IS1 family transposase -
AM483_RS17255 3292002..3292181 + 180 Protein_3183 Cys-tRNA(Pro) deacylase -
AM483_RS17260 3292205..3293005 + 801 WP_000439798.1 hypothetical protein -
AM483_RS17265 3293002..3293505 + 504 WP_000667000.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T74194 WP_000057523.1 NZ_CP020116:c3289794-3289492 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T74194 NZ_CP020116:c3289794-3289492 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT74194 WP_000806442.1 NZ_CP020116:c3289456-3289115 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT74194 NZ_CP020116:c3289456-3289115 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References