Detailed information of TA system TA074134

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1338274..1339001
• Replicon: chromosome
• Accession: NZ_CP020107
• Organism: Escherichia coli strain 13E0767

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: EC767_RS06735
• Protein ID: WP_000547564.1
• Coordinates: 1338274..1338585 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EC767_RS06740
• Protein ID: WP_000126294.1
• Coordinates: 1338582..1339001 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC767_RS06705	1333408..1335117	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
EC767_RS06710	1335127..1335669	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
EC767_RS06715	1335669..1336436	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
EC767_RS06720	1336433..1336843	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
EC767_RS06725	1336836..1337306	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
EC767_RS06730	1337349..1338100	+	752	Protein_1217	hypothetical protein	-
EC767_RS06735	1338274..1338585	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
EC767_RS06740	1338582..1339001	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
EC767_RS06745	1339115..1340539	-	1425	WP_000110313.1	6-phospho-beta-glucosidase AscB	-
EC767_RS06750	1340548..1342005	-	1458	WP_001107856.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
EC767_RS06755	1342265..1343275	+	1011	WP_001363554.1	DNA-binding transcriptional regulator AscG	-
EC767_RS06760	1343424..1343951	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T74134 WP_000547564.1 NZ_CP020107:1338274-1338585 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T74134 NZ_CP020107:1338274-1338585 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT74134 WP_000126294.1 NZ_CP020107:1338582-1339001 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT74134 NZ_CP020107:1338582-1339001 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5