Detailed information of TA system TA074129

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 277607..278209
• Replicon: chromosome
• Accession: NZ_CP020107
• Organism: Escherichia coli strain 13E0767

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: EC767_RS01410
• Protein ID: WP_000897305.1
• Coordinates: 277607..277918 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EC767_RS01415
• Protein ID: WP_000356397.1
• Coordinates: 277919..278209 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC767_RS01385	273521..274120	+	600	WP_001295269.1	glucose-1-phosphatase	-
EC767_RS01390	274114..274986	+	873	WP_000920762.1	virulence factor BrkB family protein	-
EC767_RS01395	274983..275420	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
EC767_RS01400	275465..276406	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
EC767_RS01405	276470..277378	-	909	WP_001162704.1	alpha/beta hydrolase	-
EC767_RS01410	277607..277918	+	312	WP_000897305.1	hypothetical protein	Toxin
EC767_RS01415	277919..278209	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
EC767_RS01425	278814..279032	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
EC767_RS01430	279251..279493	+	243	WP_001086388.1	hypothetical protein	-
EC767_RS01435	279722..280702	-	981	WP_000399648.1	IS110-like element IS621 family transposase	-
EC767_RS01445	281102..282031	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
EC767_RS01450	282028..282663	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	279722..280702	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T74129 WP_000897305.1 NZ_CP020107:277607-277918 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T74129 NZ_CP020107:277607-277918 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT74129 WP_000356397.1 NZ_CP020107:277919-278209 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT74129 NZ_CP020107:277919-278209 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7