Detailed information of TA system TA074117

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2410496..2410867
• Replicon: chromosome
• Accession: NZ_CP020106
• Organism: Escherichia coli strain 13E0780

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: D6I8D3
• Locus tag: EC780_RS12875
• Protein ID: WP_001307771.1
• Coordinates: 2410673..2410867 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2410496..2410674 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC780_RS12835	2405938..2406594	-	657	WP_000011652.1	carbon-nitrogen hydrolase family protein	-
EC780_RS12840	2406696..2406926	-	231	WP_000916763.1	DNA polymerase III subunit theta	-
EC780_RS12845	2407065..2407439	+	375	WP_000168747.1	CopC domain-containing protein YobA	-
EC780_RS12850	2407443..2408315	+	873	WP_000879298.1	copper homeostasis membrane protein CopD	-
EC780_RS12855	2408328..2408669	+	342	WP_000976476.1	YebY family protein	-
EC780_RS12860	2409062..2410138	-	1077	WP_001189085.1	phage integrase Arm DNA-binding domain-containing protein	-
EC780_RS12865	2410104..2410385	-	282	WP_001311878.1	excisionase	-
EC780_RS12870	2410492..2410680	-	189	WP_024165285.1	DUF1187 family protein	-
-	2410496..2410674	+	179	NuclAT_0	-	Antitoxin
-	2410496..2410674	+	179	NuclAT_0	-	Antitoxin
-	2410496..2410674	+	179	NuclAT_0	-	Antitoxin
-	2410496..2410674	+	179	NuclAT_0	-	Antitoxin
EC780_RS12875	2410673..2410867	-	195	WP_001307771.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
EC780_RS12880	2410899..2411579	-	681	WP_000186708.1	YqaJ viral recombinase family protein	-
EC780_RS12885	2411576..2412361	-	786	WP_001484907.1	phage recombination protein Bet	-
EC780_RS12890	2412367..2412663	-	297	WP_001484906.1	host-nuclease inhibitor protein Gam	-
EC780_RS12895	2412641..2414657	-	2017	Protein_2359	hypothetical protein	-
EC780_RS12900	2414759..2415034	-	276	WP_001358843.1	hypothetical protein	-
EC780_RS12905	2415109..2415285	-	177	WP_000258918.1	hypothetical protein	-
EC780_RS12910	2415279..2415500	-	222	WP_000560228.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2401916..2453522	51606

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 6986.91 Da
• Isoelectric Point: 9.6161

>T74117 WP_001307771.1 NZ_CP020106:c2410867-2410673 [Escherichia coli]
VRYTKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T74117 NZ_CP020106:c2410867-2410673 [Escherichia coli]
GTGCGATACACAAAAGTCAAACCATGCCCTTTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
CGCGAAGTGTAACGGATGCGAATCCCGAACCGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT74117 NZ_CP020106:2410496-2410674 [Escherichia coli]
GAGGACAGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTCCCCGA
GAGCATTTTTTCGCATTCCGATTTGGTTAACTTTGTTTTTGAGTACCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7H9KGA4

Antitoxin