Detailed information of TA system TA074106

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1336800..1337527
• Replicon: chromosome
• Accession: NZ_CP020106
• Organism: Escherichia coli strain 13E0780

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: EC780_RS07130
• Protein ID: WP_000547563.1
• Coordinates: 1336800..1337111 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EC780_RS07135
• Protein ID: WP_000126296.1
• Coordinates: 1337108..1337527 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC780_RS07100	1331943..1333652	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
EC780_RS07105	1333662..1334204	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
EC780_RS07110	1334204..1334971	+	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
EC780_RS07115	1334968..1335378	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
EC780_RS07120	1335371..1335841	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
EC780_RS07125	1335866..1336627	+	762	WP_001026448.1	hypothetical protein	-
EC780_RS07130	1336800..1337111	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
EC780_RS07135	1337108..1337527	+	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
EC780_RS07140	1337641..1339065	-	1425	WP_000110373.1	6-phospho-beta-glucosidase AscB	-
EC780_RS07145	1339074..1340531	-	1458	Protein_1290	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
EC780_RS07150	1340791..1341801	+	1011	WP_001343660.1	DNA-binding transcriptional regulator AscG	-
EC780_RS07155	1341950..1342477	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T74106 WP_000547563.1 NZ_CP020106:1336800-1337111 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T74106 NZ_CP020106:1336800-1337111 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAGATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT74106 WP_000126296.1 NZ_CP020106:1337108-1337527 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT74106 NZ_CP020106:1337108-1337527 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACCAAGAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22