Detailed information of TA system TA074101

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 751332..752131
• Replicon: chromosome
• Accession: NZ_CP020106
• Organism: Escherichia coli strain 13E0780

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: EC780_RS03960
• Protein ID: WP_000347274.1
• Coordinates: 751332..751796 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: EC780_RS03965
• Protein ID: WP_001307405.1
• Coordinates: 751796..752131 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC780_RS03925	746333..746767	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
EC780_RS03930	746785..747663	-	879	WP_001298758.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
EC780_RS03935	747653..748432	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
EC780_RS03940	748443..748916	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
EC780_RS03945	748939..750219	-	1281	WP_032314918.1	tagatose-bisphosphate aldolase subunit KbaZ	-
EC780_RS03955	750468..751277	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
EC780_RS03960	751332..751796	-	465	WP_000347274.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
EC780_RS03965	751796..752131	-	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
EC780_RS03970	752280..753851	-	1572	WP_001273771.1	galactarate dehydratase	-
EC780_RS03975	754226..755560	+	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
EC780_RS03980	755576..756346	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17835.22 Da
• Isoelectric Point: 9.4947

>T74101 WP_000347274.1 NZ_CP020106:c751796-751332 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLVVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T74101 NZ_CP020106:c751796-751332 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGTGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT74101 WP_001307405.1 NZ_CP020106:c752131-751796 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT74101 NZ_CP020106:c752131-751796 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0XVC7