Detailed information of TA system TA074099

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 276422..277024
• Replicon: chromosome
• Accession: NZ_CP020106
• Organism: Escherichia coli strain 13E0780

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: EC780_RS01425
• Protein ID: WP_000897305.1
• Coordinates: 276422..276733 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EC780_RS01430
• Protein ID: WP_000356397.1
• Coordinates: 276734..277024 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC780_RS01400	272336..272935	+	600	WP_001295269.1	glucose-1-phosphatase	-
EC780_RS01405	272929..273801	+	873	WP_000920762.1	virulence factor BrkB family protein	-
EC780_RS01410	273798..274235	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
EC780_RS01415	274280..275221	+	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
EC780_RS01420	275285..276193	-	909	WP_001162704.1	alpha/beta hydrolase	-
EC780_RS01425	276422..276733	+	312	WP_000897305.1	hypothetical protein	Toxin
EC780_RS01430	276734..277024	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
EC780_RS01440	277629..277847	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
EC780_RS01445	278066..278308	+	243	WP_001086388.1	hypothetical protein	-
EC780_RS01450	278537..279517	-	981	WP_000399648.1	IS110-like element IS621 family transposase	-
EC780_RS01460	279917..280846	-	930	WP_080029389.1	formate dehydrogenase accessory protein FdhE	-
EC780_RS01465	280843..281478	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	278537..279517	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T74099 WP_000897305.1 NZ_CP020106:276422-276733 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T74099 NZ_CP020106:276422-276733 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT74099 WP_000356397.1 NZ_CP020106:276734-277024 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT74099 NZ_CP020106:276734-277024 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7