Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2104786..2105288 Replicon chromosome
Accession NZ_CP020092
Organism Escherichia coli strain 13E0725

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag EC725_RS10960 Protein ID WP_000767829.1
Coordinates 2105034..2105288 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EC725_RS10955 Protein ID WP_001259255.1
Coordinates 2104786..2105037 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC725_RS10925 2099963..2101030 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
EC725_RS10930 2101030..2102100 - 1071 WP_000108962.1 histidinol-phosphate transaminase -
EC725_RS10935 2102097..2103401 - 1305 WP_001397240.1 histidinol dehydrogenase -
EC725_RS10940 2103407..2104306 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
EC725_RS10950 2104452..2104502 - 51 WP_001364200.1 his operon leader peptide -
EC725_RS10955 2104786..2105037 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EC725_RS10960 2105034..2105288 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
EC725_RS10965 2105371..2106195 + 825 WP_000754752.1 SDR family oxidoreductase -
EC725_RS10970 2106241..2107170 + 930 WP_000803351.1 LysR family transcriptional regulator -
EC725_RS27165 2107385..2107447 + 63 WP_010723108.1 membrane protein YoeI -
EC725_RS10975 2107437..2108795 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
EC725_RS10980 2108974..2110032 + 1059 WP_000492346.1 transport protein YeeE -
EC725_RS10985 2110046..2110273 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T74056 WP_000767829.1 NZ_CP020092:2105034-2105288 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T74056 NZ_CP020092:2105034-2105288 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT74056 WP_001259255.1 NZ_CP020092:2104786-2105037 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT74056 NZ_CP020092:2104786-2105037 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References