Detailed information of TA system TA074054

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1303932..1304659
• Replicon: chromosome
• Accession: NZ_CP020092
• Organism: Escherichia coli strain 13E0725

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: EC725_RS06830
• Protein ID: WP_000547563.1
• Coordinates: 1303932..1304243 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EC725_RS06835
• Protein ID: WP_000126296.1
• Coordinates: 1304240..1304659 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC725_RS06800	1299075..1300784	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
EC725_RS06805	1300794..1301336	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
EC725_RS06810	1301336..1302103	+	768	WP_033816125.1	formate hydrogenlyase subunit HycG	-
EC725_RS06815	1302100..1302510	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
EC725_RS06820	1302503..1302973	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
EC725_RS06825	1302998..1303759	+	762	WP_001026448.1	hypothetical protein	-
EC725_RS06830	1303932..1304243	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
EC725_RS06835	1304240..1304659	+	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
EC725_RS06840	1304773..1306197	-	1425	WP_000110373.1	6-phospho-beta-glucosidase AscB	-
EC725_RS06845	1306206..1307663	-	1458	Protein_1243	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
EC725_RS06850	1307923..1308933	+	1011	WP_001343660.1	DNA-binding transcriptional regulator AscG	-
EC725_RS06855	1309082..1309609	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T74054 WP_000547563.1 NZ_CP020092:1303932-1304243 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T74054 NZ_CP020092:1303932-1304243 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAGATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT74054 WP_000126296.1 NZ_CP020092:1304240-1304659 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT74054 NZ_CP020092:1304240-1304659 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACCAAGAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22