Detailed information of TA system TA074046

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5184358..5184960
• Replicon: chromosome
• Accession: NZ_CP020089
• Organism: Enterobacter cloacae strain PIMB10EC27

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5E1AN02
• Locus tag: B2J95_RS25515
• Protein ID: WP_013099360.1
• Coordinates: 5184649..5184960 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: B2J95_RS25510
• Protein ID: WP_013099359.1
• Coordinates: 5184358..5184648 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B2J95_RS25495	5181855..5182757	+	903	WP_014833793.1	formate dehydrogenase subunit beta	-
B2J95_RS25500	5182754..5183389	+	636	WP_013099357.1	formate dehydrogenase cytochrome b556 subunit	-
B2J95_RS25505	5183386..5184315	+	930	WP_029882351.1	formate dehydrogenase accessory protein FdhE	-
B2J95_RS25510	5184358..5184648	-	291	WP_013099359.1	helix-turn-helix domain-containing protein	Antitoxin
B2J95_RS25515	5184649..5184960	-	312	WP_013099360.1	hypothetical protein	Toxin
B2J95_RS25520	5185190..5186107	+	918	WP_058679010.1	alpha/beta hydrolase	-
B2J95_RS25525	5186120..5187061	-	942	WP_013099362.1	fatty acid biosynthesis protein FabY	-
B2J95_RS25530	5187106..5187543	-	438	WP_013099363.1	D-tyrosyl-tRNA(Tyr) deacylase	-
B2J95_RS25535	5187540..5188421	-	882	WP_013099364.1	virulence factor BrkB family protein	-
B2J95_RS25540	5188415..5189014	-	600	WP_013099365.1	glucose-1-phosphatase	-
B2J95_RS25545	5189134..5189934	-	801	WP_013099366.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12210.18 Da
• Isoelectric Point: 9.7550

>T74046 WP_013099360.1 NZ_CP020089:c5184960-5184649 [Enterobacter cloacae]
MLFIETDIFTEDVKTLLDDDEYHKLQVFLATQPDYGDLIQNTGGLRKIRWLSGGRGKRGGVRVIYFHRTHEFEIRLLLIY
RKGIKDDLSAREKAILKKMIERW

Nucleotide

• Length: 312 bp

>T74046 NZ_CP020089:c5184960-5184649 [Enterobacter cloacae]
ATGCTATTCATTGAAACGGATATCTTCACAGAAGACGTCAAAACGTTACTGGATGACGACGAATACCATAAGCTCCAGGT
GTTCCTGGCAACACAACCTGATTATGGCGATCTGATCCAGAATACCGGGGGGCTGCGAAAGATTCGTTGGCTTTCAGGAG
GCAGGGGAAAGCGCGGTGGAGTAAGGGTTATTTATTTTCATCGTACCCACGAGTTTGAAATCAGATTGCTGCTCATCTAT
CGCAAAGGGATCAAAGATGACCTGTCCGCGAGAGAAAAAGCCATTCTGAAAAAGATGATTGAGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10623.13 Da
• Isoelectric Point: 10.4747

>AT74046 WP_013099359.1 NZ_CP020089:c5184648-5184358 [Enterobacter cloacae]
MDKALFERLTQSIAQMDDIVGGKREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVISALSD

Nucleotide

• Length: 291 bp

>AT74046 NZ_CP020089:c5184648-5184358 [Enterobacter cloacae]
ATGGATAAGGCACTGTTTGAGCGATTAACACAAAGCATAGCTCAGATGGATGACATTGTCGGGGGGAAACGCGAGCCGTC
GCGCACCTTTCAAATCGATGCGATGAAAATCAAAGAAATTCGTCAGGCGTCTGGGTTGTCCCAAAGTAAATTCGCGAACT
TAATCTCAGTCAGCGTTGATACGTTGCGTAACTGGGAACAAGGACGGCGTTCGCCTACCGGTCCCGCAAAGGCATTGTTG
CGTGCAATTGCGAATGATCCACAGCACGTTATTTCGGCTTTATCAGATTAA

Structures

Toxin

• AlphaFold DB: A0A5E1AN02