Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4119871..4120565 Replicon chromosome
Accession NZ_CP020086
Organism Shigella flexneri 1a strain 0670

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag B4376_RS22520 Protein ID WP_001263491.1
Coordinates 4119871..4120269 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag B4376_RS22525 Protein ID WP_000554759.1
Coordinates 4120272..4120565 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B4376_RS22490 (4115210) 4115210..4115668 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
B4376_RS22495 (4115930) 4115930..4117387 + 1458 WP_001293030.1 cytosol nonspecific dipeptidase -
B4376_RS22500 (4117444) 4117444..4118004 - 561 Protein_4048 peptide chain release factor H -
B4376_RS22505 (4118120) 4118120..4119163 - 1044 WP_005053205.1 RNA ligase RtcB family protein -
B4376_RS22515 (4119409) 4119409..4119861 - 453 WP_001059860.1 GNAT family N-acetyltransferase -
B4376_RS22520 (4119871) 4119871..4120269 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
B4376_RS22525 (4120272) 4120272..4120565 - 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
B4376_RS22530 (4120617) 4120617..4121672 - 1056 WP_001226172.1 DNA polymerase IV -
B4376_RS22535 (4121743) 4121743..4122528 - 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
B4376_RS22540 (4122500) 4122500..4124212 + 1713 Protein_4055 flagellar biosynthesis protein FlhA -
B4376_RS22545 (4124429) 4124429..4124926 - 498 WP_000006251.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gtrB 4055255..4126747 71492


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T74021 WP_001263491.1 NZ_CP020086:c4120269-4119871 [Shigella flexneri 1a]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T74021 NZ_CP020086:c4120269-4119871 [Shigella flexneri 1a]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT74021 WP_000554759.1 NZ_CP020086:c4120565-4120272 [Shigella flexneri 1a]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT74021 NZ_CP020086:c4120565-4120272 [Shigella flexneri 1a]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
AGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTTCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
AAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References