Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 4009033..4009877 Replicon chromosome
Accession NZ_CP020086
Organism Shigella flexneri 1a strain 0670

Toxin (Protein)


Gene name itaT Uniprot ID -
Locus tag B4376_RS21805 Protein ID WP_014532172.1
Coordinates 4009416..4009877 (+) Length 154 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID A5H0K0
Locus tag B4376_RS21795 Protein ID WP_005053053.1
Coordinates 4009033..4009344 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B4376_RS21770 (4004504) 4004504..4005001 + 498 Protein_3914 COX aromatic rich motif-containing protein -
B4376_RS21775 (4005023) 4005023..4007014 + 1992 Protein_3915 cytochrome o ubiquinol oxidase subunit I -
B4376_RS21780 (4007004) 4007004..4007618 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
B4376_RS21785 (4007618) 4007618..4007947 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
B4376_RS21790 (4007959) 4007959..4008849 + 891 WP_094092419.1 heme o synthase -
B4376_RS21795 (4009033) 4009033..4009344 + 312 WP_005053053.1 DUF1778 domain-containing protein Antitoxin
B4376_RS21805 (4009416) 4009416..4009877 + 462 WP_014532172.1 GNAT family N-acetyltransferase Toxin
B4376_RS21815 (4010773) 4010773..4012926 + 2154 WP_005060811.1 SEL1-like repeat protein -
B4376_RS21820 (4012979) 4012979..4014709 + 1731 WP_000645013.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 4009925..4010428 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-133)

Antitoxin

Vibrio phage ICP1, Orf50

(15-97)


Sequences

Toxin        


Download         Length: 154 a.a.        Molecular weight: 16623.52 Da        Isoelectric Point: 9.9538

>T74020 WP_014532172.1 NZ_CP020086:4009416-4009877 [Shigella flexneri 1a]
MIAVTLLSINLYALRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVMLGGAR
KYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFYARLGFVQLSATPNAFGAVPMFLAIQHILAA

Download         Length: 462 bp

>T74020 NZ_CP020086:4009416-4009877 [Shigella flexneri 1a]
TTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACTG
TGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAGAC
AACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGGAGCGCGG
AAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAAAAATTCACCCGGCATTACC
AATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCGA
CACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11394.07 Da        Isoelectric Point: 10.3388

>AT74020 WP_005053053.1 NZ_CP020086:4009033-4009344 [Shigella flexneri 1a]
MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT74020 NZ_CP020086:4009033-4009344 [Shigella flexneri 1a]
ATGCCTGGAAAAACAGCCGCTCTATACGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A5H0K0

References