Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3972077..3972695 Replicon chromosome
Accession NZ_CP020086
Organism Shigella flexneri 1a strain 0670

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag B4376_RS21600 Protein ID WP_001291435.1
Coordinates 3972477..3972695 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q0T7C3
Locus tag B4376_RS21595 Protein ID WP_000344797.1
Coordinates 3972077..3972451 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B4376_RS21585 (3967166) 3967166..3968359 + 1194 WP_011069267.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
B4376_RS21590 (3968382) 3968382..3971531 + 3150 WP_001132484.1 efflux RND transporter permease AcrB -
B4376_RS21595 (3972077) 3972077..3972451 + 375 WP_000344797.1 Hha toxicity modulator TomB Antitoxin
B4376_RS21600 (3972477) 3972477..3972695 + 219 WP_001291435.1 HHA domain-containing protein Toxin
B4376_RS21605 (3972867) 3972867..3973418 + 552 Protein_3883 maltose O-acetyltransferase -
B4376_RS21610 (3973534) 3973534..3974004 + 471 WP_000136192.1 YlaC family protein -
B4376_RS21615 (3974168) 3974168..3975718 + 1551 WP_005098218.1 cyclic-guanylate-specific phosphodiesterase PdeB -
B4376_RS21620 (3975760) 3975760..3976113 - 354 WP_000878140.1 DUF1428 domain-containing protein -
B4376_RS21630 (3976492) 3976492..3976803 + 312 WP_000409911.1 MGMT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 3976833..3978161 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T74019 WP_001291435.1 NZ_CP020086:3972477-3972695 [Shigella flexneri 1a]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T74019 NZ_CP020086:3972477-3972695 [Shigella flexneri 1a]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14513.38 Da        Isoelectric Point: 4.9091

>AT74019 WP_000344797.1 NZ_CP020086:3972077-3972451 [Shigella flexneri 1a]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQALQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT74019 NZ_CP020086:3972077-3972451 [Shigella flexneri 1a]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGTTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGCTCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAACGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TPD8

References