Detailed information of TA system TA074016

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2963507..2963732
• Replicon: chromosome
• Accession: NZ_CP020086
• Organism: Shigella flexneri 1a strain 0670

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: B4376_RS16030
• Protein ID: WP_000813254.1
• Coordinates: 2963507..2963662 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2963674..2963732 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B4376_RS15985	2958650..2959132	-	483	WP_000068107.1	ORF6N domain-containing protein	-
B4376_RS15990	2959203..2960342	-	1140	WP_000088354.1	IS3 family transposase	-
B4376_RS15995	2960404..2960577	-	174	WP_000504450.1	hypothetical protein	-
B4376_RS16000	2960730..2961284	-	555	WP_000640143.1	DUF1133 family protein	-
B4376_RS16005	2961281..2961571	-	291	WP_000228038.1	DUF1364 domain-containing protein	-
B4376_RS16010	2961571..2962170	-	600	WP_000940329.1	DUF1367 family protein	-
B4376_RS16020	2962304..2963001	+	698	WP_225620329.1	IS1 family transposase	-
B4376_RS16030	2963507..2963662	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	2963674..2963732	+	59	-	-	Antitoxin
B4376_RS16040	2964117..2964482	-	366	WP_000610655.1	DUF551 domain-containing protein	-
B4376_RS16045	2964482..2965147	-	666	WP_000208062.1	hypothetical protein	-
B4376_RS16050	2965144..2965509	-	366	WP_001229297.1	HNH endonuclease signature motif containing protein	-
B4376_RS16055	2965511..2965729	-	219	WP_000256998.1	DUF4014 family protein	-
B4376_RS16060	2965822..2966178	-	357	WP_005048249.1	hypothetical protein	-
B4376_RS16065	2966236..2966658	-	423	WP_001118167.1	DUF977 family protein	-
B4376_RS16070	2966673..2967419	-	747	WP_000788996.1	ATP-binding protein	-
B4376_RS28410	2967565..2967734	-	170	Protein_2874	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	sitABCD	ipaH9.8	2905647..2976764	71117

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T74016 WP_000813254.1 NZ_CP020086:c2963662-2963507 [Shigella flexneri 1a]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T74016 NZ_CP020086:c2963662-2963507 [Shigella flexneri 1a]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT74016 NZ_CP020086:2963674-2963732 [Shigella flexneri 1a]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATTTGTGAGACATAGATTGGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin