Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 2436687..2436912 Replicon chromosome
Accession NZ_CP020086
Organism Shigella flexneri 1a strain 0670

Toxin (Protein)


Gene name hokW Uniprot ID S1ELB5
Locus tag B4376_RS12900 Protein ID WP_000813254.1
Coordinates 2436687..2436842 (-) Length 52 a.a.

Antitoxin (RNA)


Gene name sokW
Locus tag -
Coordinates 2436854..2436912 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B4376_RS12835 2431999..2432349 - 351 WP_005049241.1 IS66 family insertion sequence element accessory protein TnpB -
B4376_RS12840 2432346..2433020 - 675 WP_004967157.1 IS66-like element accessory protein TnpA -
B4376_RS12845 2433119..2433334 - 216 WP_000839572.1 class II holin family protein -
B4376_RS12875 2434130..2434818 - 689 Protein_2313 bacteriophage antitermination protein Q -
B4376_RS12880 2434815..2435180 - 366 WP_000140020.1 RusA family crossover junction endodeoxyribonuclease -
B4376_RS12885 2435181..2436239 - 1059 WP_001265248.1 DUF968 domain-containing protein -
B4376_RS12890 2436241..2436519 - 279 WP_011069426.1 hypothetical protein -
B4376_RS12900 2436687..2436842 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD Toxin
- 2436854..2436912 + 59 - - Antitoxin
B4376_RS12915 2437494..2437910 - 417 WP_005069274.1 hypothetical protein -
B4376_RS12920 2437937..2438077 + 141 Protein_2319 DUF4224 domain-containing protein -
B4376_RS12925 2438077..2439127 + 1051 Protein_2320 tyrosine-type recombinase/integrase -
B4376_RS12935 2439338..2440135 + 798 WP_001325918.1 DgsA anti-repressor MtfA -
B4376_RS12945 2440473..2441735 + 1263 Protein_2322 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - ipaH9.8 2407557..2464896 57339


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(6-47)


Sequences

Toxin        


Download         Length: 52 a.a.        Molecular weight: 5736.89 Da        Isoelectric Point: 6.1531

>T74005 WP_000813254.1 NZ_CP020086:c2436842-2436687 [Shigella flexneri 1a]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Download         Length: 156 bp

>T74005 NZ_CP020086:c2436842-2436687 [Shigella flexneri 1a]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTTATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin


Download         Length: 59 bp

>AT74005 NZ_CP020086:2436854-2436912 [Shigella flexneri 1a]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TMW0

Antitoxin

Download structure file

References