Detailed information of TA system TA073997

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1542532..1543093
• Replicon: chromosome
• Accession: NZ_CP020085
• Organism: Glaesserella parasuis strain CL120103

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A143CE91
• Locus tag: B4X03_RS07480
• Protein ID: WP_005712614.1
• Coordinates: 1542815..1543093 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: B4X03_RS07475
• Protein ID: WP_005712616.1
• Coordinates: 1542532..1542804 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B4X03_RS07460	1539048..1539590	+	543	WP_021113523.1	single-stranded DNA-binding protein	-
B4X03_RS07465	1539751..1541238	-	1488	WP_082259251.1	DUF853 domain-containing protein	-
B4X03_RS07470	1541248..1542468	-	1221	WP_026916465.1	ATP-dependent RNA helicase RhlB	-
B4X03_RS07475	1542532..1542804	-	273	WP_005712616.1	HigA family addiction module antitoxin	Antitoxin
B4X03_RS07480	1542815..1543093	-	279	WP_005712614.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B4X03_RS07485	1543242..1544252	+	1011	WP_082259253.1	ornithine carbamoyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-81)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10326.71 Da
• Isoelectric Point: 10.0505

>T73997 WP_005712614.1 NZ_CP020085:c1543093-1542815 [Glaesserella parasuis]
MIISFKHKGLKLFFETGSTAGIQANHASKLAFQLATLNQAKSALDMNAPSWNLHQLQGNLAGHWSIKVSGNWRLTFKFQN
GHAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T73997 NZ_CP020085:c1543093-1542815 [Glaesserella parasuis]
ATGATTATTTCATTTAAGCATAAAGGATTGAAATTATTTTTTGAAACAGGATCAACTGCAGGTATTCAGGCAAATCACGC
TAGTAAACTTGCTTTTCAATTAGCCACTCTAAACCAAGCAAAAAGTGCTTTAGATATGAATGCACCTAGCTGGAATTTAC
ATCAATTACAAGGTAATCTAGCTGGGCATTGGTCTATCAAGGTAAGTGGAAATTGGCGTTTAACTTTTAAATTTCAAAAT
GGTCACGCTGAAGTCGTGGACTATCAAGATTATCATTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10314.06 Da
• Isoelectric Point: 9.8982

>AT73997 WP_005712616.1 NZ_CP020085:c1542804-1542532 [Glaesserella parasuis]
MKMYNPAHPGLILREYIEGYNVTEVAKRLGVTRVTLSRILNGKAAISPEMALRLSQLLKTSPELWLGLQSQYDLWQLEQR
PSFNIQPLFA

Nucleotide

• Length: 273 bp

>AT73997 NZ_CP020085:c1542804-1542532 [Glaesserella parasuis]
ATGAAAATGTACAATCCAGCTCACCCAGGGCTTATTTTAAGAGAATATATTGAAGGATATAATGTCACAGAAGTGGCTAA
ACGTTTAGGTGTCACTCGTGTCACGCTTTCTCGTATTTTAAATGGCAAAGCTGCAATTTCACCTGAAATGGCTCTTAGAT
TAAGCCAATTATTAAAAACTAGCCCAGAATTATGGCTTGGTTTACAATCCCAATACGATCTTTGGCAGCTTGAACAACGC
CCAAGTTTTAATATTCAGCCGTTATTTGCATAG

Structures

Toxin

• AlphaFold DB: A0A143CE91