Detailed information of TA system TA073939

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 117011..117735
• Replicon: plasmid unitig_1
• Accession: NZ_CP020062
• Organism: Klebsiella pneumoniae strain AR_0117

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2J4YLV3
• Locus tag: AM364_RS26925
• Protein ID: WP_023292165.1
• Coordinates: 117424..117735 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM364_RS26920
• Protein ID: WP_020801939.1
• Coordinates: 117011..117427 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM364_RS26865	112357..112665	-	309	WP_071599372.1	hypothetical protein	-
AM364_RS26870	112725..113546	-	822	WP_020804822.1	DUF945 domain-containing protein	-
AM364_RS26890	114372..115205	-	834	WP_042922549.1	type I restriction-modification system subunit M	-
AM364_RS26895	115254..115400	-	147	WP_004152750.1	hypothetical protein	-
AM364_RS26900	115495..115842	-	348	WP_020804846.1	hypothetical protein	-
AM364_RS26905	115909..116289	-	381	WP_020802391.1	hypothetical protein	-
AM364_RS26920	117011..117427	-	417	WP_020801939.1	helix-turn-helix domain-containing protein	Antitoxin
AM364_RS26925	117424..117735	-	312	WP_023292165.1	type II toxin-antitoxin system HigB family toxin	Toxin
AM364_RS26930	117842..118066	-	225	WP_042922612.1	hypothetical protein	-
AM364_RS26935	118077..118283	-	207	WP_042922554.1	hypothetical protein	-
AM364_RS26950	118920..119270	-	351	WP_004152758.1	hypothetical protein	-
AM364_RS30110	119267..119539	-	273	WP_042922555.1	hypothetical protein	-
AM364_RS26960	119897..120619	-	723	WP_020804573.1	plasmid SOS inhibition protein A	-
AM364_RS26965	120616..121047	-	432	WP_020802174.1	conjugation system SOS inhibitor PsiB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaSHV-5 / sul1 / qacE / catB3 / ant(2'')-Ia / dfrA19	-	1..152697	152697

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-133)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12497.32 Da
• Isoelectric Point: 9.9242

>T73939 WP_023292165.1 NZ_CP020062:c117735-117424 [Klebsiella pneumoniae]
VHVISRAPFDEAARHYPNDAAAIDDTYRVLKRVVAKKPDELKRYFTSLDRMKYREKWWVIDIGGNNLRMMFFADFERGKI
FVKHITTHAEYDRLTKYYREHKE

Nucleotide

• Length: 312 bp

>T73939 NZ_CP020062:c117735-117424 [Klebsiella pneumoniae]
GTGCATGTTATATCACGAGCCCCTTTTGATGAGGCGGCGAGACACTATCCGAATGATGCTGCGGCCATCGATGACACCTA
TCGTGTACTAAAGCGGGTTGTCGCAAAAAAACCGGATGAGCTAAAGCGATACTTTACTAGTCTGGATCGCATGAAATACC
GGGAAAAATGGTGGGTTATCGATATCGGTGGTAATAACCTTAGAATGATGTTTTTTGCTGATTTTGAGCGCGGAAAGATA
TTCGTCAAACACATAACCACCCATGCTGAATATGATCGCTTAACGAAATATTATCGGGAGCACAAAGAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15380.49 Da
• Isoelectric Point: 4.5542

>AT73939 WP_020801939.1 NZ_CP020062:c117427-117011 [Klebsiella pneumoniae]
MIFSDAIKAANDLASIVPLLGGSSSRKDYEEALKLVEYLLEHDPDSPLVDMLTARIDAWEDNAVEFEEFNTRIEAGKNGV
SLLRVLMQQHGLSQSDFENEIGKKSLVSRILSGERSLTLDHMRALANRFQIPVSMFVG

Nucleotide

• Length: 417 bp

>AT73939 NZ_CP020062:c117427-117011 [Klebsiella pneumoniae]
ATGATTTTTAGCGATGCCATTAAAGCGGCGAATGACCTGGCGAGTATTGTCCCTCTGCTGGGTGGCAGCTCGTCCCGCAA
AGATTACGAAGAGGCGCTTAAACTCGTTGAATATCTTCTGGAACATGACCCGGACAGCCCGCTGGTTGATATGCTGACTG
CCCGCATCGATGCCTGGGAAGATAACGCGGTGGAGTTTGAAGAATTTAATACGCGCATCGAAGCAGGCAAAAACGGTGTA
TCGTTGCTGCGGGTTCTTATGCAGCAACACGGGCTTTCACAGTCCGATTTTGAAAACGAGATAGGCAAAAAATCGCTCGT
CAGCCGGATCCTCAGTGGTGAACGCAGTCTGACCCTTGATCACATGCGGGCGCTGGCAAATCGATTTCAAATCCCGGTCT
CGATGTTTGTTGGCTGA

Structures

Toxin

• AlphaFold DB: A0A2J4YLV3