Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 2953422..2954019 Replicon chromosome
Accession NZ_CP020061
Organism Klebsiella pneumoniae strain AR_0117

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag AM364_RS14405 Protein ID WP_004142563.1
Coordinates 2953702..2954019 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag AM364_RS14400 Protein ID WP_004142561.1
Coordinates 2953422..2953709 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM364_RS14370 2949502..2949750 + 249 WP_020802447.1 DUF1158 domain-containing protein -
AM364_RS14375 2949768..2950109 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
AM364_RS14380 2950140..2951255 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
AM364_RS14385 2951435..2952016 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
AM364_RS14390 2952016..2952384 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
AM364_RS14395 2952504..2953157 + 654 WP_020802451.1 oxygen-insensitive NAD(P)H nitroreductase -
AM364_RS14400 2953422..2953709 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
AM364_RS14405 2953702..2954019 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AM364_RS14410 2954204..2955247 - 1044 WP_020802450.1 DUF2157 domain-containing protein -
AM364_RS14420 2955914..2956780 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
AM364_RS14425 2956889..2958316 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T73930 WP_004142563.1 NZ_CP020061:c2954019-2953702 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T73930 NZ_CP020061:c2954019-2953702 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT73930 WP_004142561.1 NZ_CP020061:c2953709-2953422 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT73930 NZ_CP020061:c2953709-2953422 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACGTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References