Detailed information of TA system TA073908

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1960197..1960891
• Replicon: chromosome
• Accession: NZ_CP020058
• Organism: Escherichia coli strain AR_0061

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: AM440_RS10200
• Protein ID: WP_001263493.1
• Coordinates: 1960197..1960595 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: AM440_RS10205
• Protein ID: WP_000554757.1
• Coordinates: 1960598..1960891 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM440_RS10170	1955197..1956441	-	1245	WP_004014227.1	esterase FrsA	-
-	1955857..1955937	-	81	NuclAT_10	-	-
-	1955857..1955937	-	81	NuclAT_10	-	-
-	1955857..1955937	-	81	NuclAT_10	-	-
-	1955857..1955937	-	81	NuclAT_10	-	-
AM440_RS10175	1956533..1956991	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
AM440_RS10180	1957252..1958709	+	1458	WP_080025611.1	cytosol nonspecific dipeptidase	-
AM440_RS10185	1958766..1959287	-	522	Protein_1867	peptide chain release factor H	-
AM440_RS10190	1959286..1959489	-	204	Protein_1868	RNA ligase RtcB family protein	-
AM440_RS10195	1959735..1960187	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
AM440_RS10200	1960197..1960595	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
AM440_RS10205	1960598..1960891	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
AM440_RS10210	1960943..1961998	-	1056	WP_001226164.1	DNA polymerase IV	-
AM440_RS10215	1962069..1962854	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
AM440_RS10220	1962826..1964538	+	1713	Protein_1874	flagellar biosynthesis protein FlhA	-
AM440_RS10225	1964762..1965259	-	498	WP_000006242.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T73908 WP_001263493.1 NZ_CP020058:c1960595-1960197 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T73908 NZ_CP020058:c1960595-1960197 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT73908 WP_000554757.1 NZ_CP020058:c1960891-1960598 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT73908 NZ_CP020058:c1960891-1960598 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6