Detailed information of TA system TA073897

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 132690..133347
• Replicon: plasmid unitig_2
• Accession: NZ_CP020056
• Organism: Escherichia coli strain AR_0069

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: AM448_RS24715
• Protein ID: WP_000270043.1
• Coordinates: 132690..133040 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM448_RS24720
• Protein ID: WP_000124640.1
• Coordinates: 133045..133347 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM448_RS24675	129164..129661	-	498	WP_000062185.1	hypothetical protein	-
AM448_RS24680	129664..130152	-	489	WP_001273096.1	DUF1643 domain-containing protein	-
AM448_RS24685	130249..130584	+	336	WP_000683477.1	hypothetical protein	-
AM448_RS24690	130599..131069	-	471	WP_001281821.1	hypothetical protein	-
AM448_RS24695	131062..131433	-	372	WP_000516918.1	hypothetical protein	-
AM448_RS24700	131444..131638	-	195	WP_000343597.1	hypothetical protein	-
AM448_RS24710	131979..132527	-	549	WP_001061573.1	hypothetical protein	-
AM448_RS24715	132690..133040	+	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM448_RS24720	133045..133347	+	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
AM448_RS24725	133374..133667	-	294	WP_001239998.1	hypothetical protein	-
AM448_RS24730	133756..134028	-	273	WP_001043046.1	HU family DNA-binding protein	-
AM448_RS24735	134086..134613	-	528	WP_004201083.1	thermonuclease family protein	-
AM448_RS25805	134616..134921	-	306	WP_171820680.1	hypothetical protein	-
AM448_RS24745	134833..135690	-	858	WP_001167036.1	hypothetical protein	-
AM448_RS24750	135677..135907	-	231	WP_000972665.1	hypothetical protein	-
AM448_RS24755	135907..136425	-	519	WP_000210757.1	nitrite reductase	-
AM448_RS24760	136422..136868	-	447	WP_000919343.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaNDM-1 / sul1 / qacE / aac(6')-Ib / blaCMY-6	htpB	1..136975	136975

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T73897 WP_000270043.1 NZ_CP020056:132690-133040 [Escherichia coli]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T73897 NZ_CP020056:132690-133040 [Escherichia coli]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT73897 WP_000124640.1 NZ_CP020056:133045-133347 [Escherichia coli]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT73897 NZ_CP020056:133045-133347 [Escherichia coli]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9